Changes in cytosolic cGMP and calcium in airway smooth muscle relaxed by 3-morpholinosydnonimine.
AuthorsJones KA, Lorenz RR, Warner DO, Katusic ZS, Sieck GC
JournalAm J Physiol
PubMed ID8304472
'Nitrovasodilators relax airway smooth muscle by both guanosine 3'',5''-cyclic monophosphate (cGMP)-dependent and cGMP-independent mechanisms and by mechanisms that reduce cytosolic calcium concentration ([Ca2+]i). This study was conducted to determine the relative importance of these mechanisms in relaxation of canine tracheal smooth muscle (CTSM) induced by 3-morpholinosydnonimine (SIN-1). We measured 1) ... More
Nitric oxide preferentially stimulates auto-ADP-ribosylation of glyceraldehyde-3-phosphate dehydrogenase compared to alcohol or lactate dehydrogenase.
AuthorsDimmeler S, Brüne B
JournalFEBS Lett
PubMed ID8416805
'Recently we demonstrated that the radical nitric oxide (NO) stimulates the auto-ADP-ribosylation of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) resulting in enzyme inhibition. To further characterize this auto-ADP-ribosylation reaction we studied alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH) for comparison. Whereas auto-ADP-ribosylation of ADH was stimulated to a minor extent ... More
Peroxynitrite-mediated inhibition of DOPA synthesis in PC12 cells.
AuthorsIschiropoulos H, Duran D, Horwitz J
JournalJ Neurochem
PubMed ID7595527
'Experimental evidence has implicated oxidative stress in the development of Parkinson''s disease, amyotrophic lateral sclerosis, and other degenerative neuronal disorders. Recently, peroxynitrite, which is formed by the nearly diffusion-limited reaction of nitric oxide with superoxide, has been suggested to be a mediator of oxidant-induced cellular injury. The potential role of ... More
'Macrophage-colony stimulating factor (M-CSF) contributes to atherogenesis by regulating macrophage-derived foam cells in atherosclerotic lesions. Here we report that nitric oxide (NO) inhibits the expression of M-CSF in human vascular endothelial cells independent of guanylyl cyclase activation. The induction of M-CSF mRNA expression by either oxidized low density lipoprotein (ox-LDL) ... More
Erythro-9-(2-hydroxy-3-nonyl)adenine inhibits cyclic GMP-stimulated phosphodiesterase in isolated cardiac myocytes.
AuthorsMéry PF, Pavoine C, Pecker F, Fischmeister R
JournalMol Pharmacol
PubMed ID7623766
'Recently, an inhibitor of adenosine deaminase, erythro-9-(2-hydroxyl-3-nonyl)adenine (EHNA), was shown to selectively block the activity of purified cGMP-stimulated phosphodiesterase (PDE) (cGS-PDE, or PDE2) in human and porcine heart [J. Mol. Cell. Cardiol. 24 (Suppl. V):102 (1992)]. Because cGS-PDE was found to mediate the cGMP-induced inhibition of L-type Ca2+ current (Ica) ... More
cGMP inhibits IP3-induced Ca2+ release in intact rat megakaryocytes via cGMP- and cAMP-dependent protein kinases.
AuthorsTertyshnikova S, Yan X, Fein A
JournalJ Physiol
PubMed ID9729619
'1. Inhibition of inositol 1,4,5-trisphosphate (IP3) receptor-mediated Ca2+ release by cGMP was examined in intact rat megakaryocytes, by using a combination of single cell fluorescence microscopy to monitor intracellular free calcium ([Ca2+]i) and flash photolysis of caged second messengers. 2. Sodium nitroprusside (SNP), a nitric oxide (NO) donor, and the ... More
Spin-trapping studies of peroxynitrite decomposition and of 3-morpholinosydnonimine N-ethylcarbamide autooxidation: direct evidence for metal-independent formation of free radical intermediates.
AuthorsAugusto O, Gatti RM, Radi R
JournalArch Biochem Biophys
PubMed ID8161194
'Decomposition of peroxynitrite, the reaction product of superoxide and nitric oxide, was studied by electron paramagnetic resonance (EPR)-spin-trapping experiments with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). Proton-catalyzed decomposition of peroxynitrite at pH 7.5 resulted in the formation of the DMPO-hydroxyl radical adduct (DMPO-OH). Yields were low as DMPO-OH decomposes by direct reactions with peroxynitrite ... More
The oxidation of alpha-tocopherol in human low-density lipoprotein by the simultaneous generation of superoxide and nitric oxide.
AuthorsHogg N, Darley-Usmar VM, Wilson MT, Moncada S
JournalFEBS Lett
PubMed ID8391994
'Peroxynitrite is the product of the reaction between nitric oxide and superoxide. It is an oxidant which can also decompose to form the hydroxyl radical and nitrogen dioxide. In this report we show that a powerful oxidant with reactivity similar to that of the hydroxyl radical is formed from the ... More
Activation of a cytosolic ADP-ribosyltransferase by nitric oxide-generating agents.
AuthorsBrüne B, Lapetina EG
JournalJ Biol Chem
PubMed ID2542278
'Sodium nitroprusside is a vasodilator and an inhibitor of platelet activation. It is thought that these effects are mediated by the spontaneous release of nitric oxide and stimulation of cytosolic guanylate cyclase. We have found that sodium nitroprusside (5-200 microM) greatly increased a cytosolic ADP-ribosyltransferase that ADP-ribosylates a soluble 39-kDa ... More
Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidant-induced cell death.
AuthorsVirág L, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID9759901
'Reactive oxidant species are important mediators of tissue injury in shock, inflammation, and reperfusion injury. The actions of a number of these oxidants (e.g., hydroxyl radical and peroxynitrite, a reactive oxidant produced by the reaction of nitric oxide and superoxide) are mediated in part by the activation of the nuclear ... More
Inhibitory effect of nitric oxide on the replication of a murine retrovirus in vitro and in vivo.
AuthorsAkarid K, Sinet M, Desforges B, Gougerot-Pocidalo MA
JournalJ Virol
PubMed ID7474119
'Nitric oxide (NO) exerts microbicidal effects on a broad spectrum of pathogens, including viruses, but its antiretrovirus properties have not yet been described. The purpose of this study was to determine whether NO inhibits murine Friend leukemia virus (FV) replication in vitro and to what extent NO may play a ... More
Nitric oxide causes inactivation of the low molecular weight phosphotyrosine protein phosphatase.
AuthorsCaselli A, Camici G, Manao G, Moneti G, Pazzagli L, Cappugi G, Ramponi G
JournalJ Biol Chem
PubMed ID7929168
'The low M(r) phosphotyrosine protein phosphatase (PTPase) and Yersinia enterocolitica PTPase are inactivated by nitric oxide-generating compounds. Inorganic phosphate, a competitive inhibitor, protects the enzymes from inactivation, suggesting that the action of NO is directed to the active sites. Low M(r) PTPase from bovine liver lost two out of eight ... More
Production of hydroxyl radicals from the simultaneous generation of superoxide and nitric oxide.
AuthorsHogg N, Darley-Usmar VM, Wilson MT, Moncada S
JournalBiochem J
PubMed ID1310595
'Both nitric oxide (NO) and superoxide are generated by macrophages, neutrophils and endothelial cells. It has been postulated that the generation of these two radicals under physiological conditions can lead to the formation of peroxynitrite and (as a result of the homolytic lysis of this molecule) the production of hydroxyl ... More
Disrupted [Ca2+]i homeostasis contributes to the toxicity of nitric oxide in cultured hippocampal neurons.
AuthorsBrorson JR, Zhang H
JournalJ Neurochem
PubMed ID9349531
'Nitric oxide (NO) has been shown to be an important mediator in several forms of neurotoxicity. We previously reported that NO alters intracellular Ca2+ concentration ([Ca2+]i) homeostasis in cultured hippocampal neurons during 20-min exposures. In this study, we examine the relationship between late alterations of [Ca2+]i homeostasis and the delayed ... More
Neuroprotection of lubeluzole is mediated through the signal transduction pathways of nitric oxide.
AuthorsMaiese K, TenBroeke M, Kue I
JournalJ Neurochem
PubMed ID9003060
'Neuronal survival after ischemic injury is determined through the induction of several biological pathways. We examined whether lubeluzole, an agent efficacious in both clinical and experimental models of cerebral ischemia, modulated the signal transduction mechanisms of nitric oxide (NO), a downstream mediator of anoxic neurodegeneration. Both pretreatment [NO survival = ... More
Role of nitric oxide and superoxide anion in elimination of low metastatic human colorectal carcinomas by unstimulated hepatic sinusoidal endothelial cells.
AuthorsEdmiston KH, Shoji Y, Mizoi T, Ford R, Nachman A, Jessup JM
JournalCancer Res
PubMed ID9537259
'Human colorectal carcinoma (CRC) cell survival for the first 24 h after implantation in the hepatic sinusoid determines its potential to colonize the liver. Nearly 10-fold more highly metastatic CX-1 cells survive within the livers of nude mice 24 h after intrasplenic injection than weakly metastatic clone A cells. Because ... More
Nitric oxide regulates IL-8 expression in melanoma cells at the transcriptional level.
AuthorsAndrew PJ, Harant H, Lindley IJ
JournalBiochem Biophys Res Commun
PubMed ID7575568
'We investigated the role of nitric oxide (NO) in the expression of interleukin-8 (IL-8) in the human melanoma cell line, G361. Three NO donors, 3-morpholinosydnonimine hydrochloride (SIN-1), S-nitroso-N-acetylpenicillamine (SNAP), and S-nitroso-L-glutathione (SNOG), all caused an increase in both IL-8 protein secretion and promoter activity. Truncation of the promoter showed that ... More
Nitric oxide donation and nitrite assays in the presence of thiols and albumin as determined by Griess' and Werringloer's methods.
AuthorsRobak J, Marcinkiewicz E, Michalska Z, Gryglewski RJ
JournalPol J Pharmacol
PubMed ID9437769
'Nitric oxide (NO) or nitrite (NO2-) were assayed using the Werringloer''s method or the Griess'' method, respectively, in the presence or absence of various thiols, amino acids, or albumin. This has been done because both methods are used to determine the generation of endogenous NO from L-arginine or exogenous NO ... More
Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum.
AuthorsTao Y, Howlett A, Klein C
JournalProc Natl Acad Sci U S A
PubMed ID1352880
'Nitric oxide-releasing compounds were shown to activate an ADP-ribosyltransferase activity in the cytosol of Dictyostelium discoideum. The enzyme ADP-ribosylated a cytosolic protein of approximately 41 kDa, p41. Neither cGMP nor GTP and its analogues affected this ADP-ribosylation. p41 differs from other substrates ADP-ribosylated by cholera, pertussis, or diphtheria toxins. Treatment ... More
Kinetics and mechanism of tetrahydrobiopterin-induced oxidation of nitric oxide.
AuthorsMayer B, Klatt P, Werner ER, Schmidt K
JournalJ Biol Chem
PubMed ID7529763
'A Clark-type nitric oxide-sensitive electrode was used for electrochemical determination of NO oxidation kinetics. Reaction with molecular oxygen followed second-order rate law with respect to NO with an overall rate constant of 9.2 +/- 0.33 x 10(6) M-2 s-1. Tetrahydrobiopterin, an essential cofactor of NO synthases, was found to induce ... More
Nitric oxide production by cultured aortic endothelial cells in response to thiol depletion and replenishment.
AuthorsMurphy ME, Piper HM, Watanabe H, Sies H
JournalJ Biol Chem
PubMed ID1918052
'The requirements and influence of thiols on the production of nitric oxide (NO) were examined in cultured porcine aortic endothelial cells. NO production was diminished when cells were pretreated with thiol-depleting agents (IC50: N-ethylmaleimide, 30 microM; 1-chloro-2,4-dinitrobenzene, 200 microM; diamide, 1.5 mM; diethyl maleate, 20 mM). The depletion of glutathione ... More
Peroxynitrite increases the degradation of aconitase and other cellular proteins by proteasome.
'We report that exposure of aconitase to moderate concentrations of peroxynitrite, 3-morpholinosydnonimine (SIN-1; a superoxide- and nitric oxide-liberating substance), or hydrogen peroxide, inhibits the enzyme and enhances susceptibility to proteolytic digestion by the isolated 20 S proteasome. Exposure to more severe levels of oxidative stress, from these same agents, causes ... More
Neuronal growth cone collapse and inhibition of protein fatty acylation by nitric oxide.
AuthorsHess DT, Patterson SI, Smith DS, Skene JH
JournalNature
PubMed ID8255294
'Nitric oxide, a free-radical gas produced endogenously by several mammalian cell types, has been implicated as a diffusible intercellular messenger subserving use-dependent modification of synaptic efficacy in the mature central nervous system. It has been suggested on theoretical grounds that nitric oxide might play an analogous role during the establishment ... More
Nitroprusside and cyclic GMP stimulate Na(+)-Ca2+ exchange activity in neuronal preparations and cultured rat astrocytes.
AuthorsAsano S, Matsuda T, Takuma K, Kim HS, Sato T, Nishikawa T, Baba A
JournalJ Neurochem
PubMed ID7760023
'The effects of nitric oxide (NO)-generating agents on 45Ca2+ uptake in rat brain slices and cultured rat astrocytes were studied in the presence of monensin, which is considered to drive the Na(+)-Ca2+ exchanger in the reverse mode. Sodium nitroprusside (SNP) at > 10 microM increased monensin-stimulated Ca2+ uptake in the ... More
Factors influencing macrophage activation by muramyl peptides: inhibition of NO synthase activity by high levels of NO.
AuthorsMorin C, Fessi H, Devissaguet JP, Puisieux F, Barratt G
JournalBiochim Biophys Acta
PubMed ID7528541
'Treatment with muramyldipeptide (MDP) or a lipophilic derivative (MTP-Chol) included in nanocapsules renders macrophages cytostatic towards tumor cells. At the same time, nitric oxide (NO) synthase (EC 1.14.23) activity is induced, as determined by measurement of the two end products of the reaction (nitrite and L-citrulline). The objective of this ... More
'Preceding the onset of insulin-dependent diabetes mellitus, pancreatic islets are infiltrated by macrophages secreting interleukin-1 beta, which exerts cytotoxic and inhibitory actions on islet beta-cell insulin secretion through induction of nitric oxide (NO) synthesis. The influence of the NO donor 3-morpholinosydnonimine (SIN-1) on insulin secretion from isolated pancreatic islets in ... More
Inhibition of skeletal muscle S1-myosin ATPase by peroxynitrite.
AuthorsTiago T, Simão S, Aureliano M, Martín-Romero FJ, Gutiérrez-Merino C
JournalBiochemistry
PubMed ID16533063
'Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity, reaching 50% inhibition with 46.7 +/- 8.3 microM SIN-1 for 8.7 microM S1, that is, at a SIN-1/S1 molar ratio of approximately 5.5. The inhibition was due to the peroxynitrite produced ... More
Nitric oxide causes ADP-ribosylation and inhibition of glyceraldehyde-3-phosphate dehydrogenase.
AuthorsDimmeler S, Lottspeich F, Brüne B
JournalJ Biol Chem
PubMed ID1512218
'Nitric oxide and nitric oxide-generating agents like 3-morpholinosydnonimine (SIN-1) stimulate the mono-ADP-ribosylation of a cytosolic, 39-kDa protein in various tissues. This protein was purified from human platelet cytosol by conventional and fast protein liquid chromatography techniques. N-terminal sequence analysis identified the isolated protein as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). ... More
Ascorbate is a potent antioxidant against peroxynitrite-induced oxidation reactions. Evidence that ascorbate acts by re-reducing substrate radicals produced by peroxynitrite.
AuthorsKirsch M, de Groot H
JournalJ Biol Chem
PubMed ID10748119
'Peroxynitrite (ONOO(((-)))/ONOOH) is expected in vivo to react predominantly with CO(2), thereby yielding NO(2)(.) and CO(3) radicals. We studied the inhibitory effects of ascorbate on both NADH and dihydrorhodamine 123 (DHR) oxidation by peroxynitrite generated in situ from 3-morpholinosydnonimine N-ethylcarbamide (SIN-1). SIN-1 (150 micrometer)-mediated oxidation of NADH (200 micrometer) was ... More
Reversible conversion of nitroxyl anion to nitric oxide by superoxide dismutase.
AuthorsMurphy ME, Sies H
JournalProc Natl Acad Sci U S A
PubMed ID1961756
'Superoxide dismutase (SOD) rapidly scavenges superoxide (O2-) and also prolongs the vasorelaxant effects of nitric oxide (NO), thought to be the endothelium-derived relaxing factor. This prolongation has been ascribed to prevention of the reaction between O2- with NO. We report that SOD supports a reversible reduction of NO to NO-. ... More
Synergistic depletion of astrocytic glutathione by glucose deprivation and peroxynitrite: correlation with mitochondrial dysfunction and subsequent cell death.
AuthorsJu C, Yoon KN, Oh YK, Kim HC, Shin CY, Ryu JR, Ko KH, Kim WK
JournalJ Neurochem
PubMed ID10800942
'Previously we reported that immunostimulated astrocytes were highly vulnerable to glucose deprivation. The augmented death was mimicked by the peroxynitrite (ONOO )-producing reagent 3-morpholinosydnonimine (SIN-1). Here we show that glucose deprivation and ONOO- synergistically deplete intracellular reduced glutathione (GSH) and augment the death of astrocytes via formation of cyclosporin A-sensitive ... More
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds.
AuthorsMessmer UK, Lapetina EG, Brüne B
JournalMol Pharmacol
PubMed ID7723736
'Endogenously generated or exogenously applied nitric oxide (NO) redox species induce apoptotic cell death in murine RAW 264.7 macrophages. Activation of the inducible NO synthase by incubation of cells with a combination of lipopolysaccharide and interferon-gamma produced internucleosomal DNA fragmentation and morphological alterations, i.e., chromatin condensation, indicative of apoptotic cell ... More
Synergistic platelet antiaggregatory effects of the adenylate cyclase activator iloprost and the guanylate cyclase activating agent SIN-1 in vivo.
AuthorsSpiecker M, Darius H, Meyer J
JournalThromb Res
PubMed ID7690993
'The aim of our study was to evaluate the platelet antiaggregatory and hemodynamic effects of the stable prostacyclin analog iloprost and the NO-donor SIN-1, an active metabolite of molsidomine. The number of circulating platelets was determined in anesthetized male Wistar rats as a measure of in vivo platelet aggregation. Platelet ... More
Molecular basis of the synergistic inhibition of platelet function by nitrovasodilators and activators of adenylate cyclase: inhibition of cyclic AMP breakdown by cyclic GMP.
AuthorsMaurice DH, Haslam RJ
JournalMol Pharmacol
PubMed ID2160060
'We investigated the roles of cyclic GMP and cyclic AMP in the inhibition of rabbit platelet aggregation and degranulation by two nitrovasodilators, sodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1; the active metabolite of molsidomine), with particular reference to the synergistic interaction of these drugs with prostaglandin E1 (PGE1). Changes in platelet ... More
Spin trapping of nitric oxide by nitronylnitroxides: measurement of the activity of no synthase from rat cerebellum.
AuthorsWoldman YYu Khramtsov VV, Grigor'ev IA, Kiriljuk IA, Utepbergenov DI
JournalBiochem Biophys Res Commun
PubMed ID7518673
'Nitric oxide (NO) has been shown to be an important mediator in vasodilation, neurotransmission and cellular cytotoxicity. We investigated a new series of nitronylnitroxyl radicals (NNR) as spin traps for NO. It was found these radicals react with NO with rate constants of about 10(4) M-1c-1 forming stable iminonitroxides with ... More
S-nitroso-glutathione inhibits platelet activation in vitro and in vivo.
AuthorsRadomski MW, Rees DD, Dutra A, Moncada S
JournalBr J Pharmacol
PubMed ID1335336
'1. The effect of S-nitroso-glutathione (GSNO), a stable nitrosothiol, on platelet activation was examined in vitro and in vivo. 2. The adhesion of human platelets to fibrillar collagen and human endothelial cell monolayers was inhibited by GSNO. 3. GSNO caused a concentration-dependent inhibition of collagen-induced platelet aggregation in vitro and ... More
Elevated production of salivary nitric oxide in oral mucosal diseases.
AuthorsOhashi M, Iwase M, Nagumo M
JournalJ Oral Pathol Med
PubMed ID10478960
'Nitric oxide (NO) is known to play an important role in biological systems. In this study, we measured levels of NO in the saliva of 39 patients with oral mucosal diseases: 21 had oral lichen planus (OLP) and 18 had recurrent aphthous ulceration (RAU). NO was assayed using the Griess ... More
Homocysteine antagonism of nitric oxide-related cytostasis in Salmonella typhimurium.
AuthorsDe Groote MA, Testerman T, Xu Y, Stauffer G, Fang FC
JournalScience
PubMed ID8602531
'Nitric oxide (NO) is associated with broad-spectrum antimicrobial activity of particular importance in infections caused by intracellular pathogens. An insertion mutation in the metL gene of Salmonella typhimurium conferred specific hypersusceptibility to S-nitrosothiol NO-donor compounds and attenuated virulence of the organism in mice. The metL gene product catalyzes two proximal ... More
AuthorsWhite R, Crow J, Spear N, Thomas S, Patel R, Green I, Beckman J, Darley-Usmar V
JournalMethods Mol Biol
PubMed ID10907007
Nitric oxide: a new paradigm for second messengers.
AuthorsKerwin JF, Lancaster JR, Feldman PL
JournalJ Med Chem
PubMed ID7473563
A vascular smooth muscles nitric oxide relaxation by a mechanism distinct of calcium changes.
AuthorsChabaud F, Danna M, Bény JL
JournalLife Sci
PubMed ID7514709
Prostaglandin F2 alpha contracts coronary arteries smooth muscles under conditions of extra cellular and intracellular calcium depletion. In these conditions, nitrogen-oxide-containing vasodilators or natural EDRF(s) released by the kinins, substance P and bradykinin, from the endothelium relax strips of pig coronary arteries. This indicates that nitric oxide not only needs ... More
Inactivation of ribonucleotide reductase by nitric oxide.
AuthorsLepoivre M, Fieschi F, Coves J, Thelander L, Fontecave M
JournalBiochem Biophys Res Commun
PubMed ID1652957
Ribonucleotide reductase has been demonstrated to be inhibited by NO synthase product(s). The experiments reported here show that nitric oxide generated from sodium nitroprusside, S-nitrosoglutathione and the sydnonimine SIN-1 inhibits ribonucleotide reductase activity present in cytosolic extracts of TA3 mammary tumor cells. Stable derivatives of these nitric oxide donors were ... More
Effect of 3-morpholinosydnonimine (SIN-1), NG-nitro-L-arginine (NNA) and NG-monomethyl-L-arginine (NMMA) on isolated anaphylactic guinea-pig hearts.
AuthorsPallapies D, Thelen K, Dembinska-Kiec A, Simmet T, Peskar BA
JournalAgents Actions Suppl
PubMed ID1632288
In isolated perfused ovalbumin-sensitized guinea-pig hearts modulating effects of nitric oxide (NO) on cardiac function and eicosanoid release were investigated. While the NO-donor SIN-1 exhibited a protective effect during cardiac anaphylaxis, inhibition of NO biosynthesis by NNA or NMMA aggravated anaphylactic changes of cardiac functions. Exogenous and endogenous NO seems ... More
Oxidative DNA damage induced by simultaneous generation of nitric oxide and superoxide.
AuthorsInoue S, Kawanishi S
JournalFEBS Lett
PubMed ID7664890
Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O2-), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (.OH) scavengers inhibited the increase of 8-OH-dG by SIN-1 or peroxynitrite. Incubation of ... More
Photoactivation of the nitric oxide donor SIN-1.
AuthorsUllrich T, Oberle S, Abate A, Schröder H
JournalFEBS Lett
PubMed ID9109387
This study investigates whether nitric oxide (NO) release from linsidomine (SIN-1), the active metabolite of the antianginal drug molsidomine, is light sensitive. A 9-h irradiation with polychromatic visible light increased the formation of nitrite (NO index metabolite) from SIN-1 (1 mM) by 61% as compared to control samples incubated in ... More
Loss of alpha-tocopherol upon exposure to nitric oxide or the sydnonimine SIN-1.
Authorsde Groot H, Hegi U, Sies H
JournalFEBS Lett
PubMed ID8417969
SIN-1 which spontaneously decomposes to yield nitric oxide (NO.) and superoxide anion (O2.-) radicals caused a loss of microsomal alpha-tocopherol paralleled by the formation of alpha-tocopheryl quinone. The loss was partially prevented by superoxide dismutase but not by catalase. The SIN-1-induced loss of alpha-tocopherol also occurred when tocopherol was dissolved ... More
Effects of nitric oxide on glutamate-gated channels and other ionic channels.
AuthorsFagni L, Bockaert J
JournalJ Chem Neuroanat
PubMed ID8811428
Nitric oxide is an endogenous molecule that plays a role of second messenger in the central and peripheral nervous system. A major action of this molecule is to control ionic channel activity. Because of technical difficulties to use nitric oxide as a gaseous compound, nitric oxide donors are often utilized ... More
Inhibition and ADP-ribose pyrophosphatase-I by nitric-oxide-generating systems: a mechanism linking nitric oxide to processes dependent on free ADP-ribose.
Rat liver ADP-ribose pyrophosphatase-I (ADPRibase-I; EC 3.6.1.13) hydrolyzes ADP-ribose with high specificity and a low Km. Thus it can participate in the control of free ADP-ribose and nonenzymatic ADP-ribosylation of proteins. Here we show that ADPRibase-I was inactivated by acidified nitrite, whereas sodium nitroprusside (SNP) or 3-morpholinosydnonimine (SIN-1) at pH ... More
Base modification and strand breakage in isolated calf thymus DNA and in DNA from human skin epidermal keratinocytes exposed to peroxynitrite or 3-morpholinosydnonimine.
AuthorsSpencer JP, Wong J, Jenner A, Aruoma OI, Cross CE, Halliwell B
JournalChem Res Toxicol
PubMed ID8902271
Exposure of isolated calf thymus DNA and human skin epidermal keratinocytes to peroxynitrite or the peroxynitrite generator, 3-morpholinosydnonimine (SIN-1), led to extensive DNA base modification. Large increases in xanthine and hypoxanthine, possible deamination products of guanine and adenine, respectively, and in 8-nitroguanine were observed, but only small changes in some ... More
A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide/cGMP pathway.
We have established a rapid, homogeneous, cell-based, and highly sensitive assay for guanosine 3'-5'-cyclic monophosphate (cGMP) that is suitable for fully automated ultra-high-throughput screening. In this assay system, cGMP production is monitored in living cells via Ca2+ influx through the olfactory cyclic nucleotide-gated cation channel CNGA2, acting as the intracellular ... More
Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment.
AuthorsMohr S, Stamler JS, Brüne B
JournalJ Biol Chem
PubMed ID8626764
Nitric oxide (NO)-related activity has been associated with an NAD+-dependent modification of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). However, the mechanism by which NO effects covalent attachment of nucleotide and its role in regulation of enzyme activity are controversial. Recent studies have shown that S-nitrosylation of GAPDH (Cys149) initiates subsequent ... More
Nitric oxide triggers a switch to growth arrest during differentiation of neuronal cells.
AuthorsPeunova N, Enikolopov G
JournalNature
PubMed ID7536899
Arrest of cell division is a prerequisite for cells to enter a program of terminal differentiation. Mitogenesis and cytostasis of neuronal cell precursors can be induced by the same or by different growth or trophic factors. Response of PC12 cells to nerve growth factor (NGF) involves a proliferative phase that ... More
Stimulation of immediate early gene expression in striatal neurons by nitric oxide.
AuthorsMorris BJ
JournalJ Biol Chem
PubMed ID7559590
Exposure of primary cultures of embryonic rat striatal neurons to agents releasing nitric oxide (NO), including sin-1 molsidomine, S-nitroso-n-acetyl-penicillamine (SNAP), and S-nitrosoglutathione, resulted in an increase in the levels of expression of the immediate early genes c-fos and zif/268 in the cultured neurons. The membrane-permeable cGMP analogue, 8-bromo-cGMP, did not ... More
A new fluorescence method using the dye FM1-43 was used to examine exocytotic release from hippocampal synaptosomes. Nitric oxide caused a marked transient stimulation of vesicle release. Several structurally unrelated nitric oxide donors, sodium nitroprusside, S-nitroso-N-acetylpenicillamine, 3-morpholino-sydnonimine, and acidified sodium nitrite, were effective. Release stimulated by nitric oxide and KCl ... More
Decreased intraocular pressure induced by nitric oxide donors is correlated to nitrite production in the rabbit eye.
AuthorsBehar-Cohen FF, Goureau O, D'Hermies F, Courtois Y
JournalInvest Ophthalmol Vis Sci
PubMed ID8675417
PURPOSE: To evaluate the effect of intraocular administration of nitric oxide (NO) donors in the rabbit eye on intraocular pressure (IOP), inflammation, and toxicity. METHODS: Intravitreal and intracameral injections of two NO donors, SIN-1 and SNAP, and SIN-1C and BSS were performed. Clinical examination, IOP measurements, protein evaluation in aqueous ... More
Formation of peroxynitrite from reaction of nitroxyl anion with molecular oxygen.
AuthorsKirsch M, de Groot H
JournalJ Biol Chem
PubMed ID11799109
Peroxynitrite (ONOO(-)/ONOOH) is generally expected to be formed in vivo from the diffusion-controlled reaction between superoxide (O(2)) and nitric oxide ((*)NO). In the present paper we show that under aerobic conditions the nitroxyl anion (NO(-)), released from Angeli's salt (disodium diazen-1-ium-1,2,2-triolate, (-)ON=NO(2)(-)), generated peroxynitrite with a yield of about 65%. ... More
Detection of protein oxidation in rat-1 fibroblasts by fluorescently labeled tyramine.
Authorsvan der Vlies D, Wirtz KW, Pap EH
JournalBiochemistry
PubMed ID11425304
Oxidative damage to proteins has been postulated as a major cause of various degenerative diseases including the loss of functional capacity during aging. A prominent target for oxidation by reactive oxygen species (ROS) is the tyrosine residue. Here we present a highly sensitive method for the detection of tyrosyl radical ... More
Nitroxides increase the detectable amount of nitric oxide released from endothelial cells.
Nitroxides are known to exert superoxide dismutase-mimetic properties and to decrease O-2- and H2O2-mediated cytotoxicity. However, the effect of nitroxides on .NO homeostasis has not been studied yet. The present study investigates the effect of nitroxides on the detectable amount of .NO released by 3-morpholinosydnonimine (SIN-1) and cultured endothelial cells. ... More
Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.
AuthorsLustig HS, von Brauchitsch KL, Chan J, Greenberg DA
JournalJ Neurochem
PubMed ID1431900
Neural injury due to ischemia and related insults is thought to involve the action of excitatory amino acids at N-methyl-D-aspartate receptors, which results in the influx of extracellular Ca2+ and the generation of nitric oxide. Because ethanol inhibits physiologic responses to excitatory amino acids, we examined its effect on toxicity ... More
Concurrent generation of nitric oxide and superoxide damages surfactant protein A.
AuthorsHaddad IY, Crow JP, Hu P, Ye Y, Beckman J, Matalon S
JournalAm J Physiol
PubMed ID7943250
The conditions under which nitric oxide (.NO) may modulate or promote lung injury have not been identified. We hypothesized that .NO-induced injury results from peroxynitrite, formed by the reaction of .NO with superoxide. The simultaneous generation of .NO and superoxide by 3-morpholinosydnonimine (SIN-1, 0.1-2 mM) resulted in oxidation of dihydrorhodamine, ... More
Nitric oxide-mediated inactivation of mammalian ferrochelatase in vivo and in vitro: possible involvement of the iron-sulphur cluster of the enzyme.
AuthorsFurukawa T, Kohno H, Tokunaga R, Taketani S
JournalBiochem J
PubMed ID7544575
To investigate the role of the iron-sulphur cluster in mammalian ferrochelatases, the terminal enzyme of the haem biosynthetic pathway, we examined the interaction of nitric oxide (NO) and ferrochelatase. When macrophage cell line RAW 264.7 cells were treated with interferon-gamma and lipopolysaccharide NO synthesis in the cells was stimulated, and ... More
Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures.
AuthorsBonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA
JournalProc Natl Acad Sci U S A
PubMed ID7638161
N-Methyl-D-aspartate (NMDA) receptor-mediated neurotoxicity may depend, in part, on the generation of nitric oxide (NO.) and superoxide anion (O2.-), which react to form peroxynitrite (OONO-). This form of neurotoxicity is thought to contribute to a final common pathway of injury in a wide variety of acute and chronic neurologic disorders, ... More
Genetic and redox determinants of nitric oxide cytotoxicity in a Salmonella typhimurium model.
AuthorsDe Groote MA, Granger D, Xu Y, Campbell G, Prince R, Fang FC
JournalProc Natl Acad Sci U S A
PubMed ID7604003
Paradoxically, nitric oxide (NO) has been found to exhibit cytotoxic, antiproliferative, or cytoprotective activity under different conditions. We have utilized Salmonella mutants deficient in antioxidant defenses or peptide transport to gain insights into NO actions. Comparison of three NO donor compounds reveals distinct and independent cellular responses associated with specific ... More
Protection by a manganese porphyrin of endogenous peroxynitrite-induced death of glial cells via inhibition of mitochondrial transmembrane potential decrease.
AuthorsChoi IY, Lee SJ, Ju C, Nam W, Kim HC, Ko KH, Kim WK
JournalGlia
PubMed ID10878602
In the cerebral ischemic penumbra, progressive metabolic deterioration eventually leads to death of glial cells. The exact mechanism for the death of glial cells is unclear. Here we report that under glucose-deprived conditions immunostimulated glial cells rapidly underwent death via production of large amounts of peroxynitrite. The cell-permeable Mn(III)tetrakis(N-methyl-4'-pyridyl)porphyrin (MnTMPyP) ... More
Nitric oxide decreases in vitro phagocytosis of photoreceptor outer segments by bovine retinal pigmented epithelial cells.
AuthorsBecquet F, Courtois Y, Goureau O
JournalJ Cell Physiol
PubMed ID8163566
The presence of nitric oxide synthase (NOS) in the retina, the constitutive isoform in photoreceptor outer segments and the inducible form in retinal pigmented epithelial (RPE) cells, has been demonstrated, but the role of the free radical NO produced, remains unknown. We have investigated the effect of NO on the ... More
Two distinct mechanisms of nitric oxide-mediated neuronal cell death show thiol dependency.
AuthorsGow AJ, Chen Q, Gole M, Themistocleous M, Lee VM, Ischiropoulos H
JournalAm J Physiol Cell Physiol
PubMed ID10837337
To better understand the mechanism(s) underlying nitric oxide (. NO)-mediated toxicity, in the presence and absence of concomitant oxidant exposure, postmitotic terminally differentiated NT2N cells, which are incapable of producing. NO, were exposed to PAPA-NONOate (PAPA/NO) and 3-morpholinosydnonimine (SIN-1). Exposure to SIN-1, which generated peroxynitrite in the range of 25-750 ... More
Involvement of intracellular Ca2+ stores in inhibitory effects of NO donor SIN-1 and cGMP.
AuthorsFranck H, Storr M, Puschmann A, Schusdziarra V, Allescher HD
JournalAm J Physiol
PubMed ID9655696
We investigated the role of K+ channels and intracellular Ca2+ stores in the relaxations induced by the NO donor 3-morpholinosydnonimine (SIN-1) and 8-bromo-cGMP (8-BrcGMP), 8-(4-chlorophenylthio)-cGMP (pCPT-cGMP), and alpha, beta-methylene-ATP in isolated segments of rat ileum. The inhibitory responses to SIN-1 and the cGMP analogs were not influenced by the K+ ... More
Long-lasting changes of calcium oscillations in astrocytes. A new form of glutamate-mediated plasticity.
AuthorsPasti L, Pozzan T, Carmignoto G
JournalJ Biol Chem
PubMed ID7797504
Long-term changes of synaptic strength in the central nervous system are mediated by an increase of cytosolic calcium concentration ([Ca2+]i) following activation of excitatory neurotransmitter receptors. These phenomena, which represent a possible cellular basis for learning and memory processes in eukaryotes, are believed to be restricted to neurons. Here we ... More
Mitochondrial sulfhydryl groups under oligomycin-inhibited, aging, and uncoupling conditions: beneficial influence of cardioprotective drugs.
AuthorsFuchs J, Freisleben HJ, Mainka L, Zimmer G
JournalArch Biochem Biophys
PubMed ID3178233
Uncoupling, oligomycin-inhibited, and aging/swelling conditions comprise three models for mitochondrial dysfunction. In these models, the effects of cardioprotective agents on rat heart mitochondrial membrane -SH reactivity have been studied. For -SH detection two different chromophores were used: dithionitrobenzoate (NbS2) and monobromobimane (MB). The objective of this study is to reveal ... More
Regulation of glial cell line-derived neurotrophic factor release from rat C6 glioblastoma cells.
AuthorsVerity AN, Wyatt TL, Hajos B, Eglen RM, Baecker PA, Johnson RM
JournalJ Neurochem
PubMed ID9453547
We have monitored glial cell line-derived neurotrophic factor (GDNF) secretion from rat C6 glioblastoma cells by ELISA. Representative cytokines, neurotrophins, growth factors, neuropeptides, and pharmacological agents were tested for their ability to modulate GDNF release. Whereas most factors tested had minimal effect, a 24-h treatment with fibroblast growth factor-1, -2, ... More
Biosynthesis of nitric oxide activates iron regulatory factor in macrophages.
Biosynthesis of nitric oxide (NO) from L-arginine modulates activity of iron-dependent enzymes, including mitochondrial acontiase, an [Fe-S] protein. We examined the effect of NO on the activity of iron regulatory factor (IRF), a cytoplasmic protein which modulates both ferritin mRNA translation and transferrin receptor mRNA stability by binding to specific ... More
Activation by nitric oxide of guanylate cyclase in endothelial cells from brain capillaries.
AuthorsMarsault R, Frelin C
JournalJ Neurochem
PubMed ID1353791
Endothelial cells (ECs) from brain microvessels respond to exogenous nitric oxide (NO) donor molecules (N-ethoxycarbonyl-3-morpholinosydnonimine and sodium nitroprusside) with large (greater than 15-fold) increases in cyclic GMP (cGMP) levels. Comparable actions of sodium nitroprusside were observed in vascular smooth muscle cells and in neuroblastoma cells. Coculturing brain capillary ECs in ... More
Inhibition of hepatic gluconeogenesis by nitric oxide: a comparison with endotoxic shock.
AuthorsHorton RA, Ceppi ED, Knowles RG, Titheradge MA
JournalBiochem J
PubMed ID8192661
Isolated hepatocytes incubated in the presence of the NO donors S-nitroso-N-acetylpenicillamine (SNAP) and 3-morpholino-sydnonimine (SIN-1) displayed a time- and dose-dependent inhibition of glucose synthesis from lactate plus pyruvate as the substrate which correlated with NO production, but not nitrite production. Neither the parent compound of SNAP, N-acetyl-DL-penicillamine (NAP), nor nitrite ... More
Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major.
AuthorsAssreuy J, Cunha FQ, Epperlein M, Noronha-Dutra A, O'Donnell CA, Liew FY, Moncada S
JournalEur J Immunol
PubMed ID8125136
Murine macrophages can be activated to produce nitric oxide (NO) and superoxide and these two radicals can react to form peroxynitrite, a powerful oxidant which may be involved in parasite killing. We now show that murine macrophages activated with zymosan and interferon-gamma (ZYM/IFN-gamma) produced both superoxide (peaking 1-2 h after ... More
Astrocytes prevent neuronal death induced by reactive oxygen and nitrogen species.
AuthorsTanaka J, Toku K, Zhang B, Ishihara K, Sakanaka M, Maeda N
JournalGlia
PubMed ID10533053
Reactive oxygen and nitrogen species (RO/NS) such as nitric oxide (NO), hydroxyl radical (OH.), and superoxide anion (O(2)(-)) are generated in a variety of neuropathological processes and damage neurons. In the present study, we investigated the neuroprotective effects of rat astrocytes against RO/NS-induced damage using neuron-glia cocultures, and the effects ... More
Differential modulation of prostaglandin H synthase-2 by nitric oxide-related species in intact cells.
Nitrogen monoxide (NO) has been reported to both activate and inhibit prostaglandin (PG) biosynthesis. This apparent paradox might be explained by the production/action of distinct NO-related species formed as a result of the prevailing redox states of different cellular systems. As such, the effect of NO donors with different redox ... More
Modulation of iron regulatory protein functions. Further insights into the role of nitrogen- and oxygen-derived reactive species.
AuthorsBouton C, Raveau M, Drapier JC
JournalJ Biol Chem
PubMed ID8567693
Iron regulatory protein (IRP) is a cytosolic bifunctional [Fe-S] protein which exhibits aconitase activity or binds iron responsive elements (IREs) in untranslated regions of specific mRNA. The modulators of these activities are the intracellular concentration of iron and, as recently described, NO synthase activity. In this study, we attempted to ... More
Hyperpermeability of intestinal epithelial monolayers is induced by NO: effect of low extracellular pH.
AuthorsUnno N, Menconi MJ, Smith M, Aguirre DE, Fink MP
JournalAm J Physiol
PubMed ID9176198
Nitric oxide (NO.) increases the permeability of Caco-2BBe enterocytic monolayers. Many of the toxic effects of NO. are thought to be mediated by the peroxynitrite anion (ONOO-), which, under mildly acidic conditions, can rearrange to yield an intermediate with reactivity similar to toxic OH.. Accordingly, we assessed the permeability of ... More
Stimulation of p42 and p44 mitogen-activated protein kinases by reactive oxygen species and nitric oxide in hippocampus.
AuthorsKanterewicz BI, Knapp LT, Klann E
JournalJ Neurochem
PubMed ID9489720
Reactive oxygen species (ROS) have been suggested to act as cellular messengers that mediate signal transduction cascades in various cell types. However, little is known about their role in this capacity in the nervous system. We have begun to investigate the role of ROS, and that of nitric oxide (NO), ... More
Mechanism of covalent modification of glyceraldehyde-3-phosphate dehydrogenase at its active site thiol by nitric oxide, peroxynitrite and related nitrosating agents.
AuthorsMohr S, Stamler JS, Brüne B
JournalFEBS Lett
PubMed ID8034046
Previous studies have suggested that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) undergoes covalent modification of an active site thiol by a NO.-induced [32P]NAD(+)-dependent mechanism. However, the efficacy of GAPDH modification induced by various NO donors was found to be independent of spontaneous rates of NO. release. To further test the validity of this ... More
Detection of superoxide and peroxynitrite in model systems and mitochondria by the luminol analogue L-012.
AuthorsDaiber A, Oelze M, August M, Wendt M, Sydow K, Wieboldt H, Kleschyov AL, Munzel T
JournalFree Radic Res
PubMed ID15129734
In the present study we investigated the specificity and sensitivity of the chemiluminescence (CL) dye and luminol analogue 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4-(2H,3H) dione (L-012) to detect reactive oxygen species (ROS) such as superoxide, peroxynitrite and hydrogen peroxide in cell free systems as well as in isolated mitochondria. The results obtained by L-012 were ... More
NO accounts completely for the oxygenated nitrogen species generated by enzymic L-arginine oxygenation.
AuthorsMülsch A, Vanin A, Mordvintcev P, Hauschildt S, Busse R
JournalBiochem J
PubMed ID1281408
We have assessed the stoichiometry of the nitric oxide (NO) synthase reaction by using a novel e.p.r. technique. NO generated by crude and partially purified NO synthase from endothelial cells and Escherichia coli-lipopolysaccharide-activated macrophages was trapped by a ferrous diethyldithiocarbamate complex dispersed in yeast. The paramagnetic ferrous mononitrosyl dithiocarbamate complex ... More
In-vitro bronchorelaxing effects of novel nitric oxide donors GEA 3268 and GEA 5145 in guinea-pigs and rats.
AuthorsVaali K, Li L, Redemann B, Paakkari I, Vapaatalo H
JournalJ Pharm Pharmacol
PubMed ID9004196
Endogenously released nitric oxide (NO) in airways might contribute to physiological bronchodilation; induced production of NO might play a role in the pathogenesis of asthma, although it could also be a compensatory mechanism to other factors that cause bronchoconstriction or inflammation. To investigate the efficacy of NO donors on bronchial ... More
Nitric oxide attenuates vascular smooth muscle cell activation by interferon-gamma. The role of constitutive NF-kappa B activity.
AuthorsShin WS, Hong YH, Peng HB, De Caterina R, Libby P, Liao JK
JournalJ Biol Chem
PubMed ID8626684
Atherogenesis involves cellular immune responses and altered vascular smooth muscle cell (SMC) function. Cytokines such as interleukin (IL)-1 alpha and interferon-gamma (IFN-gamma) may contribute to this process by activating SMC. To determine whether the anti-atherogenic mediator, nitric oxide (.NO), can modulate cytokine-induced SMC activation, we investigated the effects of various ... More
Nitroglycerin-induced tolerance affects multiple sites in the organic nitrate bioconversion cascade.
AuthorsHenry PJ, Horowitz JD, Louis WJ
JournalJ Pharmacol Exp Ther
PubMed ID2563771
The effect of nitroglycerin (NTG)-induced tolerance on the spasmolytic effects of a series of vasodilators was determined to establish potential sites of tolerance. Concentration-effect curves to vasodilators were completed concurrently in U46619-contracted bovine isolated coronary artery rings pre-exposed to 100 microM NTG for 10 min (NTG-tolerant rings) and in control ... More
Phosphorylation of focal adhesion vasodilator-stimulated phosphoprotein at Ser157 in intact human platelets correlates with fibrinogen receptor inhibition.
AuthorsHorstrup K, Jablonka B, Hönig-Liedl P, Just M, Kochsiek K, Walter U
JournalEur J Biochem
PubMed ID7925440
Integrins and other adhesion receptors are essential components for outside-in and inside-out signaling through the cell membrane. The platelet glycoprotein IIb-IIIa (also known as fibrinogen receptor or integrin alpha IIb beta 3) is activated by platelet agonists, inhibited by cyclic-nucleotide-elevating agents, and is involved in the activation of protein tyrosine ... More
Regional specific effects of nitric oxide donors and cGMP on the electrical activity of neurons in the rat spinal cord.
AuthorsSchmid HA, Pehl U
JournalJ Chem Neuroanat
PubMed ID8811423
Numerous functional studies establish the role of nitric oxide (NO) as a neuromodulator in the central nervous system which affects synaptic transmission. However, there are only a few reports indicating a direct and postsynaptic effect of nitric oxide on the electrical activity of neurons in the central nervous system. The ... More
Possible mechanism of bronchoprotection by SIN-1 in anaesthetized guinea pigs: roles of nitric oxide and peroxynitrite.
AuthorsKanazawa H, Hirata K, Yoshikawa J
JournalClin Exp Allergy
PubMed ID10691906
BACKGROUND: S-morpholinosydnonimine (SIN-1) is thought to generate peroxynitrite. Recent reports suggested that peroxynitrite possessed a potent vascular relaxant activity via guanylate cyclase activation. However, no previous studies have examined the relaxant effect of peroxynitrite on airway smooth muscle. OBJECTIVE: To determine the mechanism of bronchoprotection by SIN-1, considering in particular ... More
Increased cytotoxicity of 3-morpholinosydnonimine to HepG2 cells in the presence of superoxide dismutase. Role of hydrogen peroxide and iron.
AuthorsGergel D, Misík V, Ondrias K, Cederbaum AI
JournalJ Biol Chem
PubMed ID7673115
3-Morpholinosydnonimine (SIN-1) is widely used to generate nitric oxide (NO(x).) and superoxide radical (O2-.). The effect of SOD on the toxicity of SIN-1 is complex, depending on what is the ultimate species responsible for toxicity. SIN-1 (< 1 mM) was only slightly toxic to HepG2 cells. Copper, zinc superoxide dismutase ... More
Nitric oxide modification of rat brain neurogranin affects its phosphorylation by protein kinase C and affinity for calmodulin.
AuthorsSheu FS, Mahoney CW, Seki K, Huang KP
JournalJ Biol Chem
PubMed ID8798403
Neurogranin (Ng) is a prominent protein kinase C (PKC) substrate which binds calmodulin (CaM) in the absence of Ca2+. Rat brain Ng contains four cysteine residues that were readily oxidized by nitric oxide (NO) donors, 1,1-diethyl-2-hydroxy-2-nitrosohydrazine (DEANO) and sodium nitroprusside, and by oxidants, H2O2 and o-iodosobenzoic acid. NO oxidation of ... More
Cyclic GMP induces oscillatory calcium signals in rat hepatocytes.
AuthorsRooney TA, Joseph SK, Queen C, Thomas AP
JournalJ Biol Chem
PubMed ID8702690
The ability of guanosine-3',5'-cyclic monophosphate (cGMP) to induce increases in the intracellular free calcium ion concentration ([Ca2+]i) was studied at the single cell level in fura-2-loaded rat hepatocytes. Both 8-bromo-cGMP (Br-cGMP) and dibutyryl cGMP (db-cGMP) produced oscillatory [Ca2+]i increases in hepatocytes. In addition, Br-cGMP increased the frequency of agonist-induced spiking ... More
Nitric oxide-induced release of acetylcholine in the nucleus accumbens: role of cyclic GMP, glutamate, and GABA.
AuthorsPrast H, Tran MH, Fischer H, Philippu A
JournalJ Neurochem
PubMed ID9648874
We have previously shown that the basal acetylcholine release in the ventral striatum is under the enhancing influence of endogenous nitric oxide (NO) and that NO donors cause pronounced increases in the acetylcholine release rate. To investigate the role of cyclic GMP, glutamate, and GABA in the NO-induced acetylcholine release, ... More
Interleukin-1 enhances pancreatic islet arachidonic acid 12-lipoxygenase product generation by increasing substrate availability through a nitric oxide-dependent mechanism.
AuthorsMa Z, Ramanadham S, Corbett JA, Bohrer A, Gross RW, McDaniel ML, Turk J
JournalJ Biol Chem
PubMed ID8557627
Interleukin-1 (IL-1) impairs insulin secretion from pancreatic islets and may contribute to the pathogenesis of insulin-dependent diabetes mellitus. IL-1 increases islet expression of nitric oxide (NO) synthase, and the resultant overproduction of NO participates in inhibition of insulin secretion because NO synthase inhibitors, e.g. NG-monomethyl-arginine (NMMA), prevent this inhibition. While ... More
Peroxynitrite modification of protein thiols: oxidation, nitrosylation, and S-glutathiolation of functionally important cysteine residue(s) in the sarcoplasmic reticulum Ca-ATPase.
AuthorsViner RI, Williams TD, Schöneich C
JournalBiochemistry
PubMed ID10493809
Skeletal muscle contraction and relaxation is efficiently modulated through the reaction of reactive oxygen-nitrogen species with sarcoplasmic reticulum protein thiols in vivo. However, the exact locations of functionally important modifications are at present unknown. Here, we determine by HPLC-MS that the modification of one (out of 24) Cys residue of ... More
Nitrogen monoxide activates iron regulatory protein 1 RNA-binding activity by two possible mechanisms: effect on the [4Fe-4S] cluster and iron mobilization from cells.
AuthorsWardrop SL, Watts RN, Richardson DR
JournalBiochemistry
PubMed ID10704227
The iron-regulatory protein 1 (IRP1) regulates the expression of several molecules involved in iron (Fe) metabolism by reversibly binding to iron-responsive elements (IREs) in the untranslated regions (UTR) of particular mRNA transcripts. Several studies have indicated that nitrogen monoxide (NO) may influence IRP1 RNA-binding activity by a direct effect on ... More
Nitric oxide inhibits capacitative Ca2+ entry by suppression of mitochondrial Ca2+ handling.
AuthorsThyagarajan B, Malli R, Schmidt K, Graier WF, Groschner K
JournalBr J Pharmacol
PubMed ID12411413
1. Nitric oxide (NO) is a key modulator of cellular Ca(2+) signalling and a determinant of mitochondrial function. Here, we demonstrate that NO governs capacitative Ca(2+) entry (CCE) into HEK293 cells by impairment of mitochondrial Ca(2+) handling. 2. Authentic NO as well as the NO donors 1-[2-(carboxylato)pyrrolidin-1-yl]diazem-1-ium-1,2-diolate (ProliNO) and 2-(N,N-diethylamino)-diazenolate-2-oxide ... More
Tyrosine modification by reactive nitrogen species: a closer look.
Authorsvan der Vliet A, Eiserich JP, O'Neill CA, Halliwell B, Cross CE
JournalArch Biochem Biophys
PubMed ID7786014
Peroxynitrite (ONOO-) is a powerful oxidant and cytotoxic species formed by the rapid reaction between nitrogen monoxide (nitric oxide, .NO) and superoxide (O2.-). At neutral pH ONOO- is partly protonated and this protonated form, peroxynitrous acid (ONOOH), decomposes rapidly to nitrate, forming (an) intermediate(s) with reactivity similar to .OH and ... More
Nitrogen monoxide decreases iron uptake from transferrin but does not mobilise iron from prelabelled neoplastic cells.
AuthorsRichardson DR, Neumannova V, Ponka P
JournalBiochim Biophys Acta
PubMed ID7766711
The effect of congeners of nitrogen monoxide (NO) on iron (Fe) uptake from 59Fe-125I-transferrin (Tf) and release of 59Fe from prelabelled cells have been investigated in SK-MEL-28 human melanoma cells, human K562 cells and mouse MDW-4 cells. These studies have been initiated as it has been suggested that the tumoricidal ... More
Bidirectional regulation of osteoclast function by nitric oxide synthase isoforms.
AuthorsBrandi ML, Hukkanen M, Umeda T, Moradi-Bidhendi N, Bianchi S, Gross SS, Polak JM, MacIntyre I
JournalProc Natl Acad Sci U S A
PubMed ID7535933
Nitric oxide (NO) produces rapid osteoclast detachment and contraction in vitro, and this effect is accompanied by a profound inhibition of bone resorption. Work by others has confirmed these findings in vivo: inhibition of NO synthase [NOS; L-arginine, NADPH: oxygen oxidoreductase (NO-forming), EC 1.14.13.39] in normal rats is followed by ... More