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Invitrogen
SSEA3 Monoclonal Antibody (MC-631)
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FIGURE: 1 / 7
SSEA3 Antibody (MA1-020) in ICC/IF
Immunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product # MA1-020), at a dilution of 1:50 for at least 1 hour at room t... View More
Product Details
MA1-020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Deer,
Human,
Mammal
Host/Isotype
Rat
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MC-631
Immunogen
Fischer rat immunized with 4-8 cell stage mouse embryos
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
potassium phosphate with 0.15M NaCl, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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