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Invitrogen
SSEA4 Monoclonal Antibody (MC-813-70), DyLight™ 650
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SSEA4 Antibody (MA1-021-D650) in ICC/IF
Immunofluorescent analysis of SSEA-4 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated SSEA-4 monoclonal antibody (Product # MA1-021-D650) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnifica... View More
产品信息
MA1-021-D650
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG3
分类
Monoclonal
类型
Antibody
克隆号
MC-813-70
抗原
human embryonal carcinoma cell line 2102Ep
偶联物
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-021-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.
靶标信息
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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