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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GATA3 Antibodies

          Invitrogen

          GATA3 Monoclonal Antibody (1A12-1D9)

          Advanced Verification
          2 Published Figures
          17 References
          View all (83) GATA3 antibodies

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          Cite GATA3 Monoclonal Antibody (1A12-1D9)

          • Antibody Testing Data (12)
          • Published Figures (2)
          • Advanced Verification (1)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          GATA3 Antibody (MA1-028) in ICC/IF

          Immunofluorescence analysis of GATA3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GATA3 Mouse Monoclonal Antibody (1A12-1D9) (Product # MA1-028) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Immunoprecipitation (IP)
          GATA3 Antibody in ChIP Assay (ChIP)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody

          Product Details

          MA1-028

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          5 µg
          View 1 publication 1 publication

          ChIP assay (ChIP)

          2.5 µg/10^6 cells
          View 1 publication 1 publication

          Affinity Purification (AP)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          C. elegans, Cynomolgus monkey, Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1A12-1D9

          Immunogen

          Purified partial recombinant human GATA3 protein expressed in E. coli.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536713

          Product Specific Information

          Western blot analysis of MA1-028 detects a prominent ~50 kDa protein in GATA3 expressing SH-SY5Y cells. The signal is enhanced in nuclear extracts due to enrichment of GATA3. This antibody has also been successfully tested in IP, IHC and Immunofluorescence applications.

          MA1-028 can be used for immunofluoresence analysis of Gata3 in human ESC lines.

          MA1-028 was successfully used in western blotting analysis of GATA3 in breast cancer cell lines.

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

          View more View less

          Gene Aliases: Gata-3; GATA3; HDR; HDRS; jal

          View more View less

          UniProt ID: (Human) P23771, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Human) 2625, (Mouse) 14462, (Rat) 85471

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate determination neuron migration type IV hypersensitivity kidney development mesonephros development lens development in camera-type eye pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling transcription from RNA polymerase II promoter defense response humoral immune response signal transduction axon guidance heart development blood coagulation negative regulation of cell proliferation male gonad development response to virus anatomical structure morphogenesis post-embryonic development organ morphogenesis positive regulation of signal transduction response to gamma radiation positive regulation of endothelial cell migration regulation of neuron projection development phosphatidylinositol 3-kinase signaling erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-4 production negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process inner ear morphogenesis response to drug regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of fat cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development digestive tract development developmental growth anatomical structure formation involved in morphogenesis negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of histone H3-K14 acetylation otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration negative regulation of DNA demethylation regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of histone H3-K9 acetylation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation cell morphogenesis cell activation positive regulation of cytokine production immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter nervous system development negative regulation of gene expression neuron differentiation T cell differentiation positive regulation of interleukin-13 production positive regulation of interleukin-5 production T cell differentiation in thymus positive regulation of cell differentiation embryonic organ development homeostasis of number of cells cellular response to cytokine stimulus renal system development
          It has to be done as per old AB suggested Products section.
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