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Invitrogen
CaMKII alpha Monoclonal Antibody (6G9)
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FIGURE: 1 / 42
CaMKII alpha Antibody (MA1-048) in WB
Western blot was performed using Anti-CaMKII alpha Monoclonal Antibody (6G9) (Product # MA1-048) and a band at ~50 kDa corresponding to CaMKII alpha was observed in Mouse and Rat Brain, Mouse and Rat Cerebellum in comparison to other tissues which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Heart (Lane 7) and Rat Heart (Lane 8) were electrophoresed using Novex® ... View More
Product Details
MA1-048
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Bacteria,
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6G9
Immunogen
Partially purified, rat CaM kinase II.
Conjugate
Form
Liquid
Concentration
1.17 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-048 detects CaM kinase II from rat and mouse tissues. This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. MA1-048 does not detect the ~60 kDa beta subunit in either phosphorylation state.
MA1-048 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing the alpha subunit of CaM kinase II. Immunofluorescence staining of CaM kinase II in rat hippocampal cells with MA1-048 results in intense staining of cell somas and neuropil areas, while nuclei are only lightly stained.
The MA1-048 immunogen is partially purified, rat CaM kinase II.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; caM-kinase II alpha chain; CaMK II; caMK-II subunit alpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI
Gene Aliases: Camk2a; CaMKII; mKIAA0968; PK2CDD; PKCCD; R74975
UniProt ID: (Mouse) P11798, (Rat) P11275
Entrez Gene ID: (Mouse) 12322, (Rat) 25400
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
GTPase activating protein binding
glutamate receptor binding
protein homodimerization activity
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
response to ischemia
protein phosphorylation
calcium ion transport
positive regulation of cardiac muscle cell apoptotic process
phosphorylation
peptidyl-serine phosphorylation
ionotropic glutamate receptor signaling pathway
angiotensin-activated signaling pathway
protein autophosphorylation
regulation of neurotransmitter secretion
regulation of neuronal synaptic plasticity
positive regulation of NF-kappaB transcription factor activity
positive regulation of calcium ion transport
regulation of mitochondrial membrane permeability involved in apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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