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Invitrogen
Dynein Monoclonal Antibody (74.1)
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图: 1 / 8
Dynein Antibody (MA1-070) in ICC/IF
Immunofluorescent analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (Product # MA1-070) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1-070
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Many,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
74.1
抗原
Purified bovine brain cytoplasmic dynein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-070 detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.
MA1-070 has been successfully used in Western blot immunofluorescence, and immunoprecipitation procedures. In Western blot analysis of HeLa cell lysate this antibody detects a ~74 kDa protein representing cytoplasmic dynein.
The MA1-070 immunogen is purified bovine brain cytoplasmic dynein.
靶标信息
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytosolic DH IC-1; DLC8; DLC8b; DNCL1; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase
基因别名: 1700064A15Rik; 6720463E02Rik; 8kDLC; C87222; CDLC2; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCLC1; DYNLL1; DYNLL2; HDLC1; Pin
UniProt ID: (Bovine) P61285, (Bovine) Q3MHR3, (Rat) P63170, (Mouse) P63168, (Rat) Q78P75, (Mouse) Q9D0M5
Entrez Gene ID: (Bovine) 404151, (Bovine) 540369, (Rat) 58945, (Mouse) 56455, (Rat) 140734, (Mouse) 68097
apoptotic process
microtubule-based process
viral process
transcription, DNA-templated
regulation of transcription, DNA-templated
nitric oxide biosynthetic process
transport
substantia nigra development
neurotransmitter metabolic process
negative regulation of phosphorylation
negative regulation of catalytic activity
regulation of catalytic activity
microtubule-based movement
intraciliary retrograde transport
negative regulation of nitric oxide biosynthetic process
cilium morphogenesis
synaptic target recognition
metabolic process
intracellular transport
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