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Invitrogen
Agrin Monoclonal Antibody (Agr 33)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-20378
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Agr 33
Immunogen
Recombinant rat agrin, C-terminal construct.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-20378 detects agrin in mouse and rat samples.
Target Information
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: extracellular matrix proteoglycan; laminin-binding domain; RP23-139J18.2; synaptic basal lamina component
Gene Aliases: AGR; Agrin; nmf380
UniProt ID: (Rat) P25304, (Mouse) A2ASQ1
Entrez Gene ID: (Rat) 25592, (Mouse) 11603
dystroglycan binding
calcium ion binding
protein binding
transmembrane receptor protein tyrosine kinase activator activity
acetylcholine receptor regulator activity
sialic acid binding
chondroitin sulfate binding
BMP binding
ATPase inhibitor activity
heparan sulfate proteoglycan binding
ion channel binding
receptor agonist activity
transforming growth factor beta binding
transporter inhibitor activity
extracellular matrix structural constituent
chondrocyte differentiation
plasma membrane organization
signal transduction
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
chemical synaptic transmission
nervous system development
synapse assembly
neuromuscular junction development
circadian rhythm
protein transport
positive regulation of Rac protein signal transduction
receptor clustering
positive regulation of neuron apoptotic process
positive regulation of synaptic growth at neuromuscular junction
positive regulation of transcription from RNA polymerase II promoter
filopodium assembly
regulation of synapse organization
regulation of cardiac muscle contraction
regulation of synaptic activity
membrane organization
regulation of microtubule cytoskeleton organization
skeletal muscle acetylcholine-gated channel clustering
regulation of cardiac muscle cell membrane potential
regulation of axon guidance
negative regulation of sodium ion export from cell
positive regulation of skeletal muscle acetylcholine-gated channel clustering
positive regulation of protein binding
positive regulation of filopodium assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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