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Invitrogen
RAN Monoclonal Antibody (ARAN1)
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图: 1 / 4
RAN Antibody (MA1-20581) in ICC/IF
Immunofluorescence analysis of RAN was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAN Monoclonal Antibody (ARAN1) (Product # MA1-20581) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilutio... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1-20581
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Hamster,
Human,
Mouse,
Rat,
Xenopus
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
ARAN1
抗原
Denatured recombinant human Ran.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
2.3 mg/mL
规格
115 µg
纯化类型
purified
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: Jurkat.
靶标信息
Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Androgen receptor-associated protein 24; ARA25; GTP-binding nuclear protein Ran; GTPase Ran; OTTHUMP00000166078; OTTHUMP00000166102; ran GTP-binding protein; RAN, member RAS oncogene family; Ras-like protein TC4; Ras-related nuclear protein
基因别名: ARA24; gsp1; I79_024397; OK/SW-cl.81; RAN; ran-1; RanTC4; Rasl2-8; tc4; XELAEV_18007741mg; xran
UniProt ID: (Bovine) Q3T054, (Rat) P62828, (Mouse) P62827, (Xenopus) P52301
Entrez Gene ID: (Bovine) 540457, (Rat) 84509, (Chinese hamster) 100769066, (Mouse) 19384, (Xenopus) 108716335
mitotic sister chromatid segregation
protein import into nucleus
protein export from nucleus
actin cytoskeleton organization
cellular protein complex localization
GTP metabolic process
cell division
snRNA import into nucleus
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
microtubule cytoskeleton organization
nucleocytoplasmic transport
mitotic nuclear division
small GTPase mediated signal transduction
spermatid development
metabolic process
positive regulation of protein binding
cellular protein localization
ribosomal subunit export from nucleus
protein import into nucleus, translocation
transport
intracellular protein transport
cell cycle
signal transduction
protein transport
regulation of protein binding
protein localization to nucleolus
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