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Invitrogen
HSF1 Monoclonal Antibody (SPM401)
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产品信息
MA1-21443
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
SPM401
抗原
Recombinant protein corresponding to residues 1-503 of mouse HSF1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.2 mg/mL
规格
100 µg
保存条件
4°C
运输条件
Wet ice
产品详细信息
MA1-21443 detects Heat Shock Factor 1 in human, mouse and rat samples.
MA1-21443 has been successfully used in gel shift, immunofluorescence, immunoprecipitation and Western blot procedures.
MA1-21443 immunogen is as recombinant protein corresponding to residues 1-503 of mouse HSF1. This antibody detects an epitope of residues 425-439.
靶标信息
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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