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Invitrogen
P-Glycoprotein Monoclonal Antibody (C219)
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FIGURE: 1 / 21
P-Glycoprotein Antibody (MA1-26528) in WB
Western blot was performed using Anti-P-Glycoprotein Monoclonal Antibody (C219) (Product # MA1-26528) and due to glycosylation, 160-230 kDa band corresponding to ABCB1 was observed across cell lines tested except MCF-7 which is reported to be negative. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Caco-2 (Lane 2), MDCK (Lane 3) and MCF-7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell Sur... View More
Product Details
MA1-26528
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Fish,
Human,
Mouse,
Rat,
Zebrafish
Published species
Dog,
Fish,
Human,
Insect,
Mouse,
Rat,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C219
Immunogen
SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Cell lines or frozen tissue, such as liver or colon.
MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (~170kDa) and MDR3 P-glycoprotein (~140kDa). In Western blot, boiling samples may reduce visibility of the protein.
For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 µg antibody for 30-60 min at 4°C.
Target Information
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABC superfamily; P-glycoprotein; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; MDR1; P-glycoprotein; MDR1A; multi-drug resistance 3; multidrug resistance p-glycoprotein; Multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 1B; Multidrug resistance protein 3; multidrug-resistance; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Phospholipid transporter ABCB1; unnamed protein product; uORF
Gene Aliases: ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; CD243; CLCS; ENPAT; Evi32; GP170; mdr; mdr-3; MDR1; Mdr1a; Mdr1b; Mdr3; p-170; P-GP; Pgp; Pgy-1; Pgy-3; PGY1; Pgy1-1; Pgy3
UniProt ID: (Human) P08183, (Mouse) P21447, (Mouse) P06795
Entrez Gene ID: (Dog) 403879, (Human) 5243, (Bovine) 281585, (Mouse) 18671, (Rat) 170913, (Mouse) 18669, (Rat) 24646
nucleotide binding
protein binding
ATP binding
xenobiotic-transporting ATPase activity
efflux transmembrane transporter activity
ATPase activity
transmembrane transporter activity
ubiquitin protein ligase binding
ATPase activity, coupled to transmembrane movement of substances
xenobiotic transporter activity
carboxylic acid transmembrane transporter activity
phosphatidylcholine-translocating ATPase activity
phosphatidylethanolamine flippase activity
ceramide floppase activity
ATPase-coupled intramembrane lipid transporter activity
floppase activity
ABC-type transporter activity
hydrolase activity
ATPase activity, coupled
transporter activity
drug transmembrane transporter activity
ATP-binding cassette (ABC) transporter
G2/M transition of mitotic cell cycle
response to hypoxia
placenta development
xenobiotic metabolic process
lipid transport
female pregnancy
lactation
circadian rhythm
response to xenobiotic stimulus
hormone transport
response to mycotoxin
response to nutrient levels
response to estradiol
response to lipopolysaccharide
response to progesterone
response to vitamin A
response to vitamin D
response to glucagon
maintenance of blood-brain barrier
cellular response to mycotoxin
daunorubicin transport
phospholipid translocation
response to cadmium ion
terpenoid transport
intestinal absorption
transmembrane transport
establishment of blood-brain barrier
transepithelial transport
cellular response to external biotic stimulus
cellular response to lipopolysaccharide
cellular response to antibiotic
cellular response to alkaloid
cellular response to tumor necrosis factor
cellular response to estradiol stimulus
cellular hyperosmotic salinity response
response to dexamethasone
cellular response to dexamethasone stimulus
stem cell proliferation
response to thyroxine
response to alcohol
response to antineoplastic agent
ceramide translocation
export across plasma membrane
transport across blood-brain barrier
response to L-glutamate
protein localization to bicellular tight junction
response to glycoside
negative regulation of sensory perception of pain
cellular response to nonylphenol
positive regulation of establishment of Sertoli cell barrier
regulation of intestinal absorption
carboxylic acid transmembrane transport
cellular response to borneol
cellular response to L-glutamate
response to codeine
response to quercetin
response to cyclosporin A
drug transmembrane export
drug transport across blood-brain barrier
establishment of blood-retinal barrier
positive regulation of response to drug
regulation of chloride transport
transport
drug transmembrane transport
drug transport
xenobiotic transport
drug export
negative regulation of cell death
metabolic process
response to organic cyclic compound
response to drug
cellular response to organic cyclic compound
establishment of endothelial blood-brain barrier
response to ionizing radiation
response to arsenic-containing substance
response to steroid hormone
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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