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Invitrogen
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MA1-26834 detects Histone H3-P from human samples.
MA1-26834 has been successfully used in flow cytometry, ELISA, immunofluorescence, dot blot, immunocytochemistry and Western blot procedures. In immunocytochemistry applications, dilute sample in KB buffer (10 mM Tris HCl, pH 7.6, 150 mM NaCl, 0.1% Triton X-100, 1% BSA).
The MA1-26834 immunogen is a synthetic phosphopeptide, conjugated to KLH, corresponding to residues A(1) R T K Q T A R K S(p) T G G K A P R K(18) of human Histone H3.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。