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Invitrogen
Complement C3b Monoclonal Antibody (755)
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Complement C3b Antibody (MA1-40155) in WB
Western blot was performed using Anti-Complement C3b Monoclonal Antibody (755) (Product # MA1-40155) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 in comparison to HEK-293 which is reported to be negative. A band around 70 kDa was also observed in Hep G2 treated with LPS and PTI which can be the processed form of complement C3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by protein transport inhibitor (1X for 4 hr) (Lane 2) and HEK-293 (Lane 3) were ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1-40155
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Fungi
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
755
抗原
Native C3
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.1 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
PBS with 0.1% BSA
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-40155 detects C3/C3b from human samples.
MA1-40155 has been successfully used in ELISA and Western blot applications. In western blot, the band size is ~190 kDa under non-reducing and ~100 kDa under reducing conditions.
The MA1-40155 immunogen is native C3.
Positive control: Human serum; Negative control: C3 deficient serum
靶标信息
Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Complement C3; iC3b
基因别名: C3; CPAMD1
Entrez Gene ID: (Human) 718
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