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Invitrogen
MBD1 Monoclonal Antibody (100B272.1)
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FIGURE: 1 / 5
MBD1 Antibody (MA1-41025) in ICC/IF
Immunocytochemistry analysis of MBD1 in NIH3T3 cells. Samples were incubated with MBD1 monoclonal antibody (Product # MA1-41025) using a dilution of 2 µg/mL overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
MA1-41025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
100B272.1
Immunogen
Synthetic peptide corresponding to amino acids 391-405 of human MBD1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
Immunogen sequence: SESEDGAGSP PPYRR
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CXXC-type zinc finger protein 3; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1; protein containing MBD 1; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription; unnamed protein product
Gene Aliases: CXXC3; MBD1; PCM1; RFT
UniProt ID: (Human) Q9UIS9, (Mouse) Q9Z2E2
Entrez Gene ID: (Human) 4152, (Mouse) 17190
DNA methylation
transcription, DNA-templated
regulation of transcription, DNA-templated
negative regulation of gene expression
negative regulation of astrocyte differentiation
negative regulation of transcription from RNA polymerase II promoter
methylation-dependent chromatin silencing
transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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