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Invitrogen
Rb (Cleaved DRb-p70) Monoclonal Antibody (172C1094)
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Rb (Cleaved DRb-p70) Antibody (MA1-41186) in WB
Western blot analysis of Rb (Cleaved DRb-p70) in lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. Samples were incubated with Rb (Cleaved DRb-p70) monoclonal antibody (Product # MA1-41186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. PVDF membrane. Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1-41186
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
172C1094
抗原
A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
PBS with 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Suggested positive control: camptothecin-treated HL-60 (24 hr).
靶标信息
The retinoblastoma gene product (Rb) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, Rb has been shown to play a role in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments (2-4). Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cleaved Rb; delta RB-p70; DRb-p70; p105-Rb; p110-RB1; pp105; pp110; pRb; Rb; Rb-p70; Retinoblastoma-associated protein
基因别名: pRb; Rb; Rb-1; RB1
UniProt ID: (Mouse) P13405
Entrez Gene ID: (Mouse) 19645
core promoter binding
RNA polymerase II activating transcription factor binding
DNA binding
protein binding
transcription factor binding
enzyme binding
kinase binding
ubiquitin protein ligase binding
identical protein binding
phosphoprotein binding
chromatin/chromatin-binding, or -regulatory protein
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of protein kinase activity
cell cycle
cell cycle arrest
regulation of mitotic cell cycle
negative regulation of cell proliferation
chromatin modification
neuron differentiation
sister chromatid biorientation
neuron projection development
maintenance of mitotic sister chromatid cohesion
glial cell apoptotic process
skeletal muscle cell differentiation
neuron maturation
enucleate erythrocyte differentiation
regulation of lipid kinase activity
myoblast differentiation
positive regulation of macrophage differentiation
negative regulation of cell cycle
positive regulation of mitotic metaphase/anaphase transition
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
cell morphogenesis involved in neuron differentiation
negative regulation of epithelial cell proliferation
striated muscle cell differentiation
cell division
neuron apoptotic process
regulation of cell cycle
protein localization to chromosome, centromeric region
cellular response to xenobiotic stimulus
regulation of cohesin loading
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
hepatocyte apoptotic process
negative regulation of hepatocyte apoptotic process
negative regulation of G1/S transition of mitotic cell cycle
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