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Invitrogen
HIV1 Tat Monoclonal Antibody (N1)
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FIGURE: 1 / 1
HIV1 Tat Antibody (MA1-71512) in ChIP
Chromatin immunoprecipitation analysis of HIV1 Tat was performed using cross-linked chromatin from Human 5A8 J-lat T lymphocytes culture latently infected with HIV1 and treated with 10 µg/mL PHA (phytohemaglutin) for 0, 4, and 8 hours. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Tat monoclonal antibody (Product # MA1-71512). Chromatin aliquots from ~1 x 105 cells were used per ChIP p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-71512
Product Specifications
Species Reactivity
Virus
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
N1
Immunogen
Recombinant Tat protein of HIV-1 BH10 purified from E. coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with HIV-1 Tat HXB2, BH10, IIIB and HAN strains. It has been mapped to amino acids 71-81 (KQPASQPRGDP - B subtype a.a. consensus).
MA1-71512 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat.
This clone may also be referred to as N1 (Tat N1).
Target Information
HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HIV-1 Tat
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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