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Invitrogen
Rhodopsin Monoclonal Antibody (1D4)
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FIGURE: 1 / 12
Rhodopsin Antibody (MA1-722) in IHC (P)
Immunohistochemistry analysis of Rhodopsin showing staining in the nucleus of paraffin-embedded mouse retinal tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature,... View More
Product Details
MA1-722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Bovine,
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1D4
Immunogen
Bovine rhodopsin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-722 detects rhodopsin from human, mouse and bovine retinal samples. Clone 1D4 has also been used successfully in Zebrafish. It does not recognize rhodopsin in zebrafish rods, but instead labels long double cone outer segments that express red opsin (Yin, J. et al, 2012).
MA1-722 has been successfully used in Western blot, IHC (P), immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
The MA1-722 immunogen is bovine rhodopsin. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4
Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Bovine) P02699, (Human) P08100, (Rat) P51489, (Mouse) P15409
Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Rat) 24717, (Mouse) 212541
G-protein alpha-subunit binding
opsin binding
guanyl-nucleotide exchange factor activity
11-cis retinal binding
protein binding
G-protein coupled photoreceptor activity
zinc ion binding
identical protein binding
arrestin family protein binding
G-protein coupled receptor activity
photoreceptor activity
metal ion binding
retinal binding
spectrin binding
signal transducer activity
G-protein coupled receptor
protein phosphorylation
visual perception
phototransduction, visible light
response to light stimulus
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
photoreceptor cell maintenance
retina development in camera-type eye
microtubule cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
phototransduction
detection of light stimulus
response to light intensity
gene expression
thermotaxis
sensory perception of light stimulus
detection of temperature stimulus involved in thermoception
cellular response to light stimulus
podosome assembly
rod bipolar cell differentiation
red, far-red light phototransduction
response to stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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