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          • Primary Antibodies ›
          • GSK3B Antibodies

          Invitrogen

          GSK3B Monoclonal Antibody (21A)

          1 Reference
          View all (47) GSK3B antibodies

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          Cite GSK3B Monoclonal Antibody (21A)

          Additional Information:
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          • Antibody Testing Data (2)
          GSK3B Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          GSK3B Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          GSK3B Antibody (MA1-7621) in WB

          Knockout of GSK3B was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR933148_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of GSK3B was performed by loading 60 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2), HeLa GSK3B KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GSK3B Antibody in Western Blot (WB)
          GSK3B Antibody in Western Blot (WB)

          Product Details

          MA1-7621

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          Flow Cytometry (Flow)

          Assay-dependent
          -

          ELISA (ELISA)

          1:1,000
          -

          Immunoprecipitation (IP)

          5-10 µg
          -
          Product Specifications

          Species Reactivity

          Human, Rat, Xenopus

          Published species

          Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          21A

          Immunogen

          Purified recombinant Xenopus laevis protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.2

          Contains

          0.01% sodium azide

          Storage conditions

          4°C short term, -80°C long term

          RRID

          AB_1074718

          Product Specific Information

          MA1-7621 detects GSK3 Beta from human, rat, and Xenopus laevis samples.

          MA1-7621 has been successfully used in ELISA, Western blot, Flow cytometry, Immunoprecipitation and Immunohistochemistry procedures.

          The MA1-7621 immunogen is a purified recombinant Xenopus laevis protein.

          Store at 4°C short term. For extended storage, keep at -80°C.

          Target Information

          GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3B; intracellular kinase; kinase; pp37; Serine/threonine-protein kinase GSK3B; unnamed protein product; Xgsk-3 protein

          View more View less

          Gene Aliases: gsk-3; gsk3; gsk3-beta; GSK3B; gsk3beta; XELAEV_180112401mg; Xgsk-3

          View more View less

          UniProt ID: (Human) P49841, (Rat) P18266, (Xenopus) Q91757

          View more View less

          Entrez Gene ID: (Human) 2932, (Rat) 84027, (Xenopus) 399097

          View more View less

          Function(s)
          nucleotide binding protease binding p53 binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding beta-catenin binding kinase activity transferase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding dynactin binding tau protein binding tau-protein kinase activity NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding scaffold protein binding protein serine kinase activity RNA polymerase II transcription factor binding integrin binding transcription factor binding ionotropic glutamate receptor binding non-receptor serine/threonine protein kinase
          Process(es)
          G1/S transition of mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion cytoplasmic translational initiation heart valve development glycogen metabolic process protein phosphorylation ER overload response mitochondrion organization regulation of mitotic cell cycle nervous system development circadian rhythm insulin receptor signaling pathway negative regulation of signal transduction positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of neuron projection development Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity cell differentiation positive regulation of cell migration negative regulation of cell migration regulation of axon extension SCF-dependent proteasomal ubiquitin-dependent protein catabolic process neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein ubiquitination negative regulation of TOR signaling positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction cellular response to interleukin-3 TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of translation positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus rhythmic process regulation of dendrite morphogenesis regulation of axonogenesis positive regulation of protein kinase B signaling canonical Wnt signaling pathway excitatory postsynaptic potential regulation of microtubule cytoskeleton organization negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand presynaptic modulation of chemical synaptic transmission neuron projection organization membraneless organelle assembly regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway cellular response to oxygen-containing compound negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity negative regulation of mesenchymal stem cell differentiation re-entry into mitotic cell cycle regulation of gene expression by genetic imprinting protein export from nucleus establishment or maintenance of cell polarity axonogenesis myoblast fusion organ morphogenesis response to lithium ion negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation phosphorylation cell migration negative regulation of protein binding positive regulation of peptidyl-serine phosphorylation protein localization to microtubule response to drug positive regulation of apoptotic process negative regulation of MAP kinase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension regulation of neuronal synaptic plasticity negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus cellular response to mechanical stimulus positive regulation of stem cell differentiation
          It has to be done as per old AB suggested Products section.

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