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Invitrogen
PPAR alpha Monoclonal Antibody (3B6/PPAR)
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PPAR alpha Antibody (MA1-822) in WB
Western blot analysis of PPAR alpha was performed by loading 25 µg of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with a PPAR alpha monoclonal antibody (Product # MA1-822) at a dilution of 1:1000 (Hela and Jurkat) and 1:500 (NIH-3T3) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr a... View More
产品信息
MA1-822
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Bovine,
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
3B6/PPAR
抗原
Purified recombinant PPAR alpha protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues.
MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates.
The MA1-822 immunogen is purified recombinant PPAR alpha protein.
靶标信息
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA; PPARalpha; unnamed protein product
基因别名: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha
UniProt ID: (Human) Q07869, (Rat) P37230, (Mouse) P23204
Entrez Gene ID: (Human) 5465, (Rat) 25747, (Mouse) 19013
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcription coactivator binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
lipid binding
phosphatase binding
protein domain specific binding
mitogen-activated protein kinase kinase kinase binding
ubiquitin conjugating enzyme binding
signaling receptor activity
sequence-specific DNA binding
macromolecular complex binding
metal ion binding
NFAT protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
MDM2/MDM4 family protein binding
DNA-binding transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II repressing transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
receptor activity
drug binding
protein complex binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
gluconeogenesis
regulation of transcription, DNA-templated
lipid metabolic process
fatty acid metabolic process
heart development
response to nutrient
lactation
epidermis development
cellular response to starvation
hormone-mediated signaling pathway
gene expression
regulation of gene expression
regulation of cellular ketone metabolic process
negative regulation of macrophage derived foam cell differentiation
negative regulation of cholesterol storage
regulation of fatty acid metabolic process
cell differentiation
negative regulation of transforming growth factor beta receptor signaling pathway
intracellular steroid hormone receptor signaling pathway
intracellular receptor signaling pathway
positive regulation of fatty acid beta-oxidation
negative regulation of appetite
response to insulin
circadian regulation of gene expression
behavioral response to nicotine
peroxisome proliferator activated receptor signaling pathway
wound healing
lipoprotein metabolic process
regulation of circadian rhythm
response to ethanol
positive regulation of gluconeogenesis
negative regulation of blood pressure
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of fatty acid metabolic process
positive regulation of transcription from RNA polymerase II promoter
nitric oxide metabolic process
positive regulation of fatty acid oxidation
positive regulation of lipid biosynthetic process
rhythmic process
negative regulation of inflammatory response
negative regulation of protein kinase B signaling
negative regulation of cell growth involved in cardiac muscle cell development
enamel mineralization
cellular response to fructose stimulus
negative regulation of cytokine production involved in inflammatory response
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of leukocyte cell-cell adhesion
negative regulation of reactive oxygen species biosynthetic process
negative regulation of hepatocyte apoptotic process
positive regulation of transformation of host cell by virus
regulation of fatty acid transport
positive regulation of ATP biosynthetic process
transcription, DNA-templated
transcription from RNA polymerase II promoter
negative regulation of receptor biosynthetic process
negative regulation of sequestering of triglyceride
negative regulation of protein binding
steroid hormone mediated signaling pathway
regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter
regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter
regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter
negative regulation of neuron death
negative regulation of transcription regulatory region DNA binding
glucose metabolic process
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