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Invitrogen
PLK1 Monoclonal Antibody (13E8)
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FIGURE: 1 / 13
PLK1 Antibody (MA1-848) in ICC/IF
Immunofluorescence analysis of PLK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLK1 Mouse monoclonal Antibody (Product # MA1-848) at 3µg/mL in 0.1% BSA and incubated for overnight at 4°C and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1... View More
Product Details
MA1-848
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
13E8
Immunogen
Recombinant protein containing residues 300-400 of human PLK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-848 detects PLK1 from human, mouse, and rat samples.
MA1-848 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures.
The MA1-848 immunogen is a recombinant protein containing residues 300-400 of human PLK1.
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; unnamed protein product
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673
Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
anaphase-promoting complex binding
kinase activity
transferase activity
protein kinase binding
identical protein binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
establishment of mitotic spindle orientation
mitotic cell cycle
nuclear division
mitotic cytokinesis
double-strand break repair via homologous recombination
microtubule bundle formation
double-strand break repair
protein phosphorylation
mitotic spindle organization
sister chromatid cohesion
mitotic chromosome condensation
mitotic nuclear envelope disassembly
metaphase/anaphase transition of mitotic cell cycle
mitotic spindle assembly checkpoint
mitotic G2 DNA damage checkpoint
centrosome cycle
regulation of mitotic cell cycle
regulation of cell cycle process
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of cytokinesis
protein localization to organelle
negative regulation of apoptotic process
homologous chromosome segregation
establishment of protein localization
positive regulation of proteolysis
Golgi inheritance
nuclear envelope disassembly
centrosome separation
cell division
positive regulation of ubiquitin-protein transferase activity
regulation of cell cycle
synaptonemal complex disassembly
protein localization to chromatin
protein localization to centrosome
protein localization to nuclear envelope
double-strand break repair via alternative nonhomologous end joining
positive regulation of protein localization to nucleus
regulation of mitotic spindle assembly
regulation of mitotic cell cycle phase transition
positive regulation of ubiquitin protein ligase activity
regulation of protein localization to cell cortex
regulation of anaphase-promoting complex-dependent catabolic process
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
cytokinesis
cell cycle
mitotic nuclear division
positive regulation of peptidyl-threonine phosphorylation
phosphorylation
G2 DNA damage checkpoint
polar body extrusion after meiotic divisions
regulation of protein binding
negative regulation of cyclin-dependent protein serine/threonine kinase activity
centrosome organization
response to antibiotic
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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