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Invitrogen
RuvB Monoclonal Antibody (4G5/1)
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Product Details
MA1-91534
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4G5/1
Immunogen
Full length protein (E. coli).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Amount
100 µg
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
Product Specific Information
MA1-91534 detects RuvB from bacteria samples.
MA1-91534 has been successfully used in radioimmune assay, immunoprecipitation, Western blot applications.
The MA1-91534 immunogen is: Full length protein (E. coli).
Target Information
In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction.during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciforms structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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