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Invitrogen
RyR1 Monoclonal Antibody (34C)
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RyR1 Antibody (MA3-925) in IHC (P)
Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal an... View More
Product Details
MA3-925
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Bovine,
Dog,
Chicken,
Fish,
Human,
Mustelid,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat
Published species
Bovine,
C. elegans,
Cat,
Chicken,
Dog,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Sheep,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
34C
Immunogen
Partially purified chicken pectoral muscle ryanodine receptor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, MA3-925 detects the alpha and beta isoforms and in fish it detects the alpha isoform.
MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.
The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.
Target Information
Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500 kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: calcium release channel; central core disease of muscle; protein phosphatase 1, regulatory subunit 137; Ryanodine receptor; Ryanodine receptor 1; ryanodine receptor 1 (skeletal); ryanodine receptor 1, skeletal muscle; ryanodine receptor 1-like, skeletal muscle; RYR-1; sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; Type 1 ryanodine receptor; type 1-like ryanodine receptor; unnamed protein product
Gene Aliases: AI528790; CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; RYR1; Ryr1l; SKRR
UniProt ID: (Rabbit) P11716, (Human) P21817, (Mouse) E9PZQ0
Entrez Gene ID: (Sheep) 101112591, (Rabbit) 100009540, (Bovine) 100139857, (Human) 6261, (Dog) 606491, (Mouse) 20190, (Rat) 114207
protease binding
ryanodine-sensitive calcium-release channel activity
voltage-gated calcium channel activity
calcium channel activity
calcium ion binding
protein binding
calmodulin binding
calcium-release channel activity
ion channel activity
enzyme binding
ligand-gated ion channel
scaffold/adaptor protein
response to hypoxia
outflow tract morphogenesis
calcium ion transport
muscle contraction
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to caffeine
ion transmembrane transport
skin development
ossification involved in bone maturation
skeletal muscle fiber development
release of sequestered calcium ion into cytosol
regulation of cytosolic calcium ion concentration
cellular response to caffeine
regulation of cardiac conduction
transport
ion transport
cellular calcium ion homeostasis
regulation of muscle contraction
multicellular organism development
regulation of ion transmembrane transport
transmembrane transport
sarcoplasmic reticulum calcium ion transport
calcium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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