Invitrogen

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

Name: Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin
Brand: Invitrogen
SKU: MA5-11544

高级验证

2个已发表图片

189 篇参考文献

查看其他47个Alpha-Smooth Muscle Actin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)

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Alpha-Smooth Muscle Actin Antibody (MA5-11544) in WB

Western blot analysis of Actin Sooth Muscle was performed by loading 25 µg of C2C12 (Lane 1), Hela (Lane 2), and A431 (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with an Actin Sooth Muscle monoclonal antibody (Product # MA5-11547) at a dilu... View More

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)

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产品信息

MA5-11544

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

查看142篇已发表文章 142篇已发表文章

免疫组化（石蜡） (IHC (P))

1:800

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

查看25篇已发表文章 25篇已发表文章

流式细胞分析 (Flow)

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Cat, Dog, Human, Lizard, Mouse, Pig, Rabbit, Rat, Rhesus monkey, Sheep

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1A4 (asm-1)

抗原

N-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

40 µg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10981631

产品详细信息

MA5-11544 targets Actin Smooth Muscle in WB, immunofluorescence and immunohistochemistry applications and shows reactivity with cow, chicken, human, mouse, non-human primate, rabbit, and rat samples.

The MA5-11544 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.

靶标信息

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; alpha actin 2; Alpha Cardiac Actin; Alpha-actin; Alpha-actin-2; alpha-cardiac actin; alpha-smooth muscle actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

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基因别名: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; asma; GIG46; MYMY5; SMalphaA