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Invitrogen
Actin Monoclonal Antibody (ACTN05 (C4))
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FIGURE: 1 / 70
Actin Antibody (MA5-11869) in ICC/IF
Immunofluorescent analysis of Actin pan (green) showing staining in the cytoplasm of T-47D cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin pan monoclonal antibody (Product # MA5-11869) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More
Product Details
MA5-11869
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Pig,
Protozoa,
Rabbit,
Rat
Published species
C. elegans,
Chicken,
Chordate,
Dog,
Ferret,
Fish,
Fruit fly,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Tag,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ACTN05 (C4)
Immunogen
Chicken gizzard actin
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.
MA5-11869 is a suggested replacement to Product # 82353, clone # C4.
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a-actin; ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; B-actin; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma 1 propeptide; gamma non-muscle actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; Bact; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GAMMA-ACTIN; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM
UniProt ID: (Human) P68133, (Bovine) P68138, (Pig) P68137, (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Rabbit) P29751, (Bovine) P60712, (Pig) Q6QAQ1, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Human) P68032, (Bovine) Q3ZC07, (Chicken) P04270, (Human) P63261, (Chicken) P53478, (Bovine) P63258, (Bovine) Q5E9B5, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Chicken) 421534, (Human) 58, (Bovine) 281592, (Rabbit) 100009506, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Chicken) 396526, (Dog) 403580, (Dog) 478250, (Human) 70, (Bovine) 533219, (Chicken) 423298, (Rabbit) 100340339, (Pig) 100152267, (Human) 71, (Chicken) 415296, (Dog) 475923, (Bovine) 404122, (Pig) 397653, (Bovine) 281595, (Chicken) 396084, (Pig) 100520667, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365
protein binding
ATP binding
structural constituent of cytoskeleton
myosin binding
ADP binding
protein kinase binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
nucleosomal DNA binding
structural constituent of postsynaptic actin cytoskeleton
ATPase activity
ubiquitin protein ligase binding
profilin binding
nucleotide binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
skeletal muscle thin filament assembly
skeletal muscle fiber development
muscle contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
positive regulation of gene expression
cell growth
muscle filament sliding
skeletal muscle fiber adaptation
response to steroid hormone
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
cell motility
apoptotic process
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
response to drug
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
actin filament organization
sarcomere organization
angiogenesis
positive regulation of cell migration
regulation of stress fiber assembly
regulation of focal adhesion assembly
positive regulation of wound healing
tight junction assembly
protein localization to bicellular tight junction
retina development in camera-type eye
cellular response to electrical stimulus
metabolic process
actin-mediated cell contraction
response to calcium ion
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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