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Invitrogen
Mitochondria Monoclonal Antibody (MTC02)
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Mitochondria Antibody (MA5-12017) in ICC/IF
Immunofluorescence analysis of Mitochondria was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Mitochondria Mouse Monoclonal Antibody (Product # MA5-12017) at 2µg/mL in 0.1% BSA and incubated for 3 hours a... View More
产品信息
MA5-12017
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Human,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MTC02
抗原
Semi-purified mitochondrial preparation
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with 0.05% BSA
内含物
0.05% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes a 60kDa non-glycosylated protein component of mitochondria found in normal and malignant human cells.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls: HeLa cells and liver tissue.
靶标信息
Antibodies that recognize subcellular organelles or compartments of human cells are useful in identification of these organelles in cells, tissues, and biochemical preparations. Anti-mitochondria antibodies can be used to stain mitochondria in cell/tissue preparations and can be used as a marker in subcellular fractions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
