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Invitrogen
S100B Monoclonal Antibody (4C4.9)
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FIGURE: 1 / 7
S100B Antibody (MA5-12969) in ICC/IF
Immunofluorescent analysis of S100 Protein (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a S100 Protein monoclonal antibody (Product # MA5-12969) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a D... View More
Product Details
MA5-12969
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4C4.9
Immunogen
Purified bovine brain S100 protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12969 targets S100 Protein in IHC (P), ICC/IF and WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.
The MA5-12969 immunogen is purified bovine brain S100 protein.
Target Information
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100 protein beta chain; S100 protein, beta polypeptide; S100B
Gene Aliases: AI850290; Bpb; NEF; S100; S100-B; S100B; S100beta
UniProt ID: (Bovine) P02638, (Human) P04271, (Mouse) P50114
Entrez Gene ID: (Bovine) 525716, (Human) 6285, (Mouse) 20203
regulation of translation
axonogenesis
learning or memory
memory
phosphorylation
negative regulation of kinase activity
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of complement activation
astrocyte activation
regulation of neuronal synaptic plasticity
negative regulation of monocyte chemotactic protein-1 production
cell adhesion
central nervous system development
positive regulation of cell proliferation
regulation of cell shape
positive regulation of myelination
positive regulation of apoptotic process
response to ethanol
positive regulation of neuron differentiation
astrocyte differentiation
positive regulation of synaptic transmission
response to glucocorticoid
response to methylmercury
long-term synaptic potentiation
cellular response to hypoxia
response to anesthetic
sympathetic neuron projection extension
neuron projection extension
adaptive thermogenesis
negative regulation of skeletal muscle cell differentiation
energy reserve metabolic process
cellular calcium ion homeostasis
regulation of cytokine biosynthetic process
regulation of long-term neuronal synaptic plasticity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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