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          • Primary Antibodies ›
          • WNT5A Antibodies

          Invitrogen

          WNT5A Monoclonal Antibody (G.307.7)

          Advanced Verification
          1 Reference
          View all (21) WNT5A antibodies

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          Cite WNT5A Monoclonal Antibody (G.307.7)

          Additional Information:

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          • Antibody Testing Data (3)
          • Advanced Verification (2)
          WNT5A Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          WNT5A Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          WNT5A Antibody (MA5-14946) in WB

          Western blot was performed using Anti-WNT5A Rabbit Monoclonal Antibody (Product # MA5-14946) and 43 kDa, 58 kDa bands corresponding to canonical WNT5A and glycosylated WNT5A respectively were observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), PANC-1 (Lane 4), HeLa (Lane 5), U-87 MG (Lane 6), U-937 (Lane 7), Hep G2 (Lane 8), Mouse Heart (Lane 9) and Rat Heart (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resol... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          WNT5A Antibody in Western Blot (WB)
          WNT5A Antibody in Western Blot (WB)
          WNT5A Antibody in Western Blot (WB)
          WNT5A Antibody
          WNT5A Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          WNT5A Monoclonal Antibody (G.307.7)

          Product Details

          MA5-14946

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          G.307.7

          Immunogen

          Synthetic peptide corresponding to residues surrounding Glu201 of Isoform 1 and Glu186 of Isoform 2 of the human Wnt5a protein
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          50 µg/mL

          Amount

          5 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986099

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody works to detect both isoforms of human Wnt5a protein.

          Target Information

          Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays an important role in cell communication and development. Wnt5A acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is suspected that Wnt5A is a developmental protein. Wnt5A is a signaling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein; hWNT5A; OTTHUMP00000212837; OTTHUMP00000212861; unnamed protein product; wingless-related MMTV integration site 5A; wingless-type MMTV integration site 5A; wingless-type MMTV integration site family, member 5A; Wnt-5a; WNT-5A protein

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          Gene Aliases: 8030457G12Rik; hWNT5A; Wnt-5a

          View more View less

          UniProt ID: (Human) P41221, (Mouse) P22725

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          Entrez Gene ID: (Human) 7474, (Mouse) 22418, (Rat) 64566

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          Function(s)
          receptor binding frizzled binding receptor tyrosine kinase-like orphan receptor binding cytokine activity protein binding phospholipid binding protein domain specific binding receptor agonist activity chemoattractant activity involved in axon guidance
          Process(es)
          ameboidal-type cell migration establishment of planar polarity somitogenesis positive regulation of cytokine production kidney development epithelial to mesenchymal transition neural tube closure positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart looping morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation positive regulation of cytokine production involved in immune response primary heart field specification secondary heart field specification ventricular septum development atrial septum development type B pancreatic cell development pericardium morphogenesis axis elongation protein phosphorylation inflammatory response signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway Wnt signaling pathway, calcium modulating pathway JNK cascade cell-cell signaling determination of left/right symmetry axonogenesis axon guidance axonal fasciculation hindgut morphogenesis midgut development protein localization positive regulation of cell proliferation male gonad development anterior/posterior axis specification, embryo organ morphogenesis anterior/posterior pattern specification positive regulation of endothelial cell migration positive regulation of gene expression macrophage derived foam cell differentiation positive regulation of peptidyl-threonine phosphorylation positive regulation of T cell chemotaxis positive regulation of neuron projection development Wnt signaling pathway cell migration olfactory bulb interneuron development neural tube development neurogenesis positive regulation of cell-cell adhesion neuron differentiation keratinocyte differentiation lung development embryonic limb morphogenesis negative regulation of BMP signaling pathway midbrain development positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production regulation of protein localization positive regulation of peptidyl-serine phosphorylation positive regulation of Rac protein signal transduction limb morphogenesis non-canonical Wnt signaling pathway post-anal tail morphogenesis chemoattraction of serotonergic neuron axon chemorepulsion of dopaminergic neuron axon negative regulation of fibroblast growth factor receptor signaling pathway wound healing inner ear morphogenesis embryonic digit morphogenesis positive regulation of macrophage activation negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of JUN kinase activity cell fate commitment establishment of epithelial cell apical/basal polarity negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of angiogenesis positive regulation of ossification positive regulation of endocytosis positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade development of primary male sexual characteristics negative regulation of melanin biosynthetic process positive regulation of fibroblast proliferation paraxial mesoderm formation digestive tract morphogenesis notochord morphogenesis embryonic skeletal system development genitalia development neuron projection morphogenesis negative regulation of axon extension involved in axon guidance hypophysis morphogenesis positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of inflammatory response positive regulation of dendrite morphogenesis modulation of synaptic transmission regulation of synapse organization negative chemotaxis positive regulation of small GTPase mediated signal transduction positive regulation of anagen positive regulation of protein kinase B signaling negative regulation of synapse assembly convergent extension convergent extension involved in axis elongation convergent extension involved in organogenesis uterus development cervix development vagina development canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway urinary bladder development face development positive regulation of type I interferon-mediated signaling pathway lateral sprouting involved in mammary gland duct morphogenesis tube closure mesenchymal-epithelial cell signaling negative regulation of prostatic bud formation mammary gland branching involved in thelarche epithelial cell proliferation involved in mammary gland duct elongation positive regulation of response to cytokine stimulus regulation of branching involved in mammary gland duct morphogenesis mesodermal to mesenchymal transition involved in gastrulation positive regulation of macrophage cytokine production positive regulation of cartilage development somite development secondary palate development positive regulation of thymocyte apoptotic process cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to calcium ion cellular response to interferon-gamma hematopoietic stem cell proliferation dopaminergic neuron differentiation cellular response to transforming growth factor beta stimulus negative regulation of mesenchymal cell proliferation primitive streak formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis melanocyte proliferation regulation of postsynaptic specialization assembly postsynaptic modulation of chemical synaptic transmission regulation of postsynapse organization regulation of postsynaptic cytosolic calcium ion concentration positive regulation of neuron projection arborization regulation of synaptic signaling by nitric oxide positive regulation of protein kinase C activity positive regulation of dendrite extension negative regulation of cell proliferation in midbrain midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of non-canonical Wnt signaling pathway positive regulation of apoptotic signaling pathway lens development in camera-type eye optic cup formation involved in camera-type eye development regulation of inflammatory response cellular response to retinoic acid presynapse assembly postsynapse assembly positive regulation of protein localization to synapse excitatory synapse assembly inhibitory synapse assembly cell differentiation prostate gland development response to estradiol response to testosterone estrous cycle lung alveolus development response to glucocorticoid response to hyperoxia response to quercetin
          It has to be done as per old AB suggested Products section.

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