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Invitrogen
Catenin alpha-1 Monoclonal Antibody (J.783.6)
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FIGURE: 1 / 3
Catenin alpha-1 Antibody (MA5-14986) in WB
Western blot was performed using Anti-Catenin alpha-1 Monoclonal Antibody (J.783.6)(Product # MA5-14986) and a 100kDa band corresponding to Catenin alpha-1 was observed across cell lines and tissue extracts tested except PC-3 and Daudi which is reported to be negative. A non-specific band around 28kDA has also been observed. Whole Cell Extract-WCL (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), Daudi (Lane 6), NIH/3T3 (Lane 7) and tissue extracts of Mouse Lung (Lane 8), Mouse Heart (Lane 9) an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-14986
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
J.783.6
Immunogen
Synthetic peptide corresponding to the amino-terminal sequence of human a-E-catenin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
92 µg/mL
Amount
9.2 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; alpha(E)-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin alpha 1; Catenin alpha-1; Renal carcinoma antigen NY-REN-13
Gene Aliases: 2010010M04Rik; AA517462; AI988031; Catna1; CTNNA1
UniProt ID: (Mouse) P26231
Entrez Gene ID: (Mouse) 12385
actin filament organization
cell adhesion
establishment or maintenance of cell polarity
negative regulation of neuroblast proliferation
gap junction assembly
cellular protein localization
regulation of cell proliferation
negative regulation of apoptotic process
apical junction assembly
positive regulation of smoothened signaling pathway
protein heterooligomerization
cellular response to indole-3-methanol
epithelial cell-cell adhesion
negative regulation of cell motility
negative regulation of integrin-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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