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Invitrogen
Phospho-GSK3 alpha (Ser21) Monoclonal Antibody (R.726.2)
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FIGURE: 1 / 2
Phospho-GSK3 alpha (Ser21) Antibody (MA5-15021) in WB
Western blot was performed using Phospho-GSK3 alpha (Ser21) Monoclonal Antibody (R.726.2) (Product # MA5-15021) and a 57 kDa band corresponding to Phospho-GSK3A (Ser21) was observed in cell line tested, which upon treatment with SB216763 and EGF was down regulated as seen in the experiment. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEK 293 (Lane 1) and HEK 293 treated with SB216763 (10 µM for ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
R.726.2
Immunogen
Synthetic phosphopeptide corresponding to residues around pSer21 of human GSK-3a
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686D0638; FA; Factor A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A
Gene Aliases: 2700086H06Rik; GSK3A
UniProt ID: (Rat) P18265, (Mouse) Q2NL51
Entrez Gene ID: (Rat) 50686, (Mouse) 606496
protein kinase activity
protein serine/threonine kinase activity
ATP binding
protein kinase A catalytic subunit binding
tau-protein kinase activity
nucleotide binding
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
regulation of systemic arterial blood pressure
cardiac left ventricle morphogenesis
glycogen metabolic process
regulation of gene expression by genetic imprinting
protein phosphorylation
signal transduction
nervous system development
insulin receptor signaling pathway
positive regulation of peptidyl-threonine phosphorylation
Wnt signaling pathway
cell migration
positive regulation of cAMP biosynthetic process
negative regulation of TOR signaling
cellular response to insulin stimulus
positive regulation of peptidyl-serine phosphorylation
cellular response to interleukin-3
proteasome-mediated ubiquitin-dependent protein catabolic process
hypermethylation of CpG island
positive regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
negative regulation of glucose import
negative regulation of insulin receptor signaling pathway
negative regulation of transferase activity
negative regulation of cell growth involved in cardiac muscle cell development
cellular response to lithium ion
cellular response to organic cyclic compound
positive regulation of adrenergic receptor signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of glycogen (starch) synthase activity
carbohydrate metabolic process
negative regulation of signal transduction
phosphorylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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