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Invitrogen
Aurora A Monoclonal Antibody (J.458.1)
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FIGURE: 1 / 3
Aurora A Antibody (MA5-15075) in ICC/IF
Immunofluorescent analysis of Aurora A/AIK in mitotic COS cells during metaphase, using an Aurora A/AIK monoclonal antibody (Product # MA5-15075) (red) and a Phospho-Histone H3 (Ser10) monoclonal antibody (green). DNA is labeled using a fluorescent blue dye.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15075
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
J.458.1
Immunogen
Synthetic peptide corresponding to the amino terminus of human Aurora A/AIK
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
12 µg/mL
Amount
1.2 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AURAK; aurora 2; Aurora A Kinase; aurora/IP; aurora/IPL1-like kinase; aurora/IPL1-related kinase 1; AURORA2; breast tumor-amplified kinase; EC 2.7.11.1; hARK1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; RP5-1167H4.6; serine/threonine protein kinase 15; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A; unnamed protein product
Gene Aliases: AIK; ARK1; AURA; BTAK; PPP1R47; STK15; STK6; STK7
UniProt ID: (Human) O14965
Entrez Gene ID: (Human) 6790
G2/M transition of mitotic cell cycle
meiotic spindle organization
microtubule cytoskeleton organization
mitotic cell cycle
protein phosphorylation
spindle organization
mitotic spindle organization
centrosome cycle
mitotic centrosome separation
response to wounding
regulation of G2/M transition of mitotic cell cycle
negative regulation of gene expression
peptidyl-serine phosphorylation
regulation of protein stability
negative regulation of protein binding
regulation of cytokinesis
regulation of microtubule-based process
cell cycle G2/M phase transition
positive regulation of mitotic nuclear division
positive regulation of mitotic cell cycle
regulation of centrosome cycle
protein autophosphorylation
meiotic cell cycle
cilium disassembly
positive regulation of cell cycle process
positive regulation of mitochondrial fission
liver regeneration
regulation of signal transduction by p53 class mediator
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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