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Invitrogen
p70 S6 Kinase Monoclonal Antibody (T.527.6)
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FIGURE: 1 / 4
p70 S6 Kinase Antibody (MA5-15141) in ICC/IF
Immunofluorescence analysis of RPS6KB1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with p70 S6 Kinase Monoclonal Antibody (T.527.6) (Product # MA5-15141) at 1:200 dilution in 0.1% BSA, incubated at 4... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
T.527.6
Immunogen
Synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
48 µg/mL
Amount
4.8 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; p70-S6K 1; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; S6K; S6K-beta-1; Serine/threonine-protein kinase 14A
Gene Aliases: 2610318I15Rik; 4732464A07Rik; 70kDa; AA959758; AI256796; AI314060; p70/85s6k; p70s6k; RPS6KB1; S6K1; STK14A
UniProt ID: (Mouse) Q8BSK8
Entrez Gene ID: (Mouse) 72508
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
ribosomal protein S6 kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
PDZ domain binding
peptide binding
protein phosphatase 2A binding
protein modifying enzyme
G1/S transition of mitotic cell cycle
behavioral fear response
regulation of translation
protein phosphorylation
apoptotic process
cell cycle
signal transduction
germ cell development
positive regulation of smooth muscle cell migration
phosphorylation
TOR signaling
response to insulin
cellular response to insulin stimulus
response to tumor necrosis factor
negative regulation of apoptotic process
protein kinase B signaling
positive regulation of translation
positive regulation of mitotic cell cycle
positive regulation of translational initiation
regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of skeletal muscle tissue growth
positive regulation of smooth muscle cell proliferation
cellular response to growth factor stimulus
cellular response to organic cyclic compound
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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