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Invitrogen
BRK Monoclonal Antibody (2H12B8)
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BRK Antibody (MA5-15328) in WB
Western blot was performed using Anti-BRK Monoclonal Antibody (2H12B8) (Product # MA5-15328) and a 48kDa band corresponding to BRK was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of Caco-2 (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), A549 (Lane 4), PANC-1 (Lane 5), and HepG2 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product #... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-15328
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
2H12B8
抗原
Purified recombinant fragment of human PTK6 expressed in E. Coli.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-15328 targets PTK6 in indirect ELISA, WB applications and shows reactivity with Human samples.
The MA5-15328 immunogen is purified recombinant fragment of human PTK6 expressed in E. Coli.
.
MA5-15328 detects PTK6 which has a predicted molecular weight of approximately 52kDa.
靶标信息
Breast tumor kinase (Brk) is a non-receptor protein tyrosine kinase expressed in over 60% of breast carcinoma tissue samples and breast tumor cell lines. Brk is 80% identity to Sik (Src-related intestinal kinase) and exhibits the features of a novel non-receptor tyrosine kinase, including amino terminal SH3 and SH2 domains. Low levels of Brk transcripts have been identified in some human breast tumor cell lines but not in normal breast tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ALT-PTK6; deltam5; FLJ42088
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
