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Invitrogen
HSPA4 Monoclonal Antibody (5A6)
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FIGURE: 1 / 5
HSPA4 Antibody (MA5-15578) in ICC/IF
Immunofluorescence analysis of HSPA4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA4 Monoclonal Antibody (5A6) (Product # MA5-15578) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More
Product Details
MA5-15578
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5A6
Immunogen
Purified recombinant fragment of human HSP70 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15578 targets HSP70 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15578 immunogen is purified recombinant fragment of human HSP70 expressed in E. Coli.
MA5-15578 detects HSP70 which has a predicted molecular weight of approximately 70kDa.
Target Information
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 4; Heat shock protein; Heat shock protein family H member 2; heat shock protein, 110 kDa; hsp70 RY; HSP70RY; unnamed protein product
Gene Aliases: 70kDa; AI317151; APG-2; APG2; HEL-S-5a; HS24/P52; Hsp110; hsp70; Hsp70RY; HSPA4; HSPH2; mKIAA4025; RY
UniProt ID: (Human) P34932
Entrez Gene ID: (Human) 3308, (Mouse) 15525
negative regulation of protein phosphorylation
response to heat
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
negative regulation of cell death
protein folding
response to unfolded protein
protein import into mitochondrial matrix
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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