Search
Invitrogen
DNMT3A Monoclonal Antibody (64B1446)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 10
DNMT3A Antibody (MA5-16171) in ICC/IF
Immunocytochemistry analysis of DNMT3A in Ntera2 cells. Samples were incubated with DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1:200 dilution overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16171
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Gerbil,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
64B1446
Immunogen
Bacteria expressed recombinant mouse Dnmt3a.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot, this antibody detects a band at approximately 120 kDa (predicted molecular weight: 102 kDa).
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), transfected cell lysate (WB), HeLa cells (ICC).
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AU015806; cysteine methyltransferase DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; unnamed protein product; W91664
Gene Aliases: DNMT3A2; HESJAS; M.HsaIIIA; MmuIIIA; TBRS
UniProt ID: (Human) Q9Y6K1, (Mouse) O88508
Entrez Gene ID: (Human) 1788, (Mouse) 13435
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription corepressor activity
DNA (cytosine-5-)-methyltransferase activity
protein binding
methyltransferase activity
identical protein binding
unmethylated CpG binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
lncRNA binding
protein-cysteine methyltransferase activity
DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates
negative regulation of transcription from RNA polymerase II promoter
methylation-dependent chromatin silencing
response to xenobiotic stimulus
response to toxic substance
response to ionizing radiation
response to lead ion
regulation of gene expression
neuron differentiation
chromatin silencing by small RNA
response to nutrient levels
response to estradiol
response to vitamin A
response to cocaine
hypermethylation of CpG island
response to ethanol
negative regulation of transcription, DNA-templated
cellular response to ethanol
cellular response to hypoxia
hepatocyte apoptotic process
transposable element silencing by piRNA-mediated DNA methylation
positive regulation of cellular response to hypoxia
developmental process involved in reproduction
gamete generation
spermatogenesis
post-embryonic development
heterochromatin assembly
DNA methylation involved in embryo development
cellular response to amino acid stimulus
genetic imprinting
autosome genomic imprinting
gene silencing by piRNA-directed DNA methylation
cellular response to bisphenol A
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support