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Invitrogen
MiTF Monoclonal Antibody (21D1418)
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FIGURE: 1 / 5
MiTF Antibody (MA5-16214) in ICC/IF
Immunocytochemistry analysis of MiTF in HeLa cells. Samples were incubated with MiTF monoclonal antibody (Product # MA5-16214) using a dilution of 2 µg/mL overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. Cells were imaged using a 100X objective and digitally deconvolved.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
21D1418
Immunogen
A synthetic peptide mapping to the 300-350 a.a. region.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: A375 or RAW.
Target Information
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bHLHe32; black eyed white; Class E basic helix-loop-helix protein 32; melanogenesis associated transcription factor; microphtalmia-associated transcription factor; microphthalmia; Microphthalmia-associated transcription factor; unnamed protein product
Gene Aliases: BCC2; BHLHE32; Bw; CMM8; COMMAD; Gsfbcc2; MI; MITF; MITF-A; Vit; Vitiligo; Wh; WS2; WS2A
UniProt ID: (Rat) O88368, (Mouse) Q08874
Entrez Gene ID: (Human) 4286, (Rat) 25094, (Mouse) 17342
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein dimerization activity
E-box binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of gene expression
melanocyte differentiation
negative regulation of cell migration
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
developmental pigmentation
protein-containing complex assembly
positive regulation of DNA-templated transcription, initiation
regulation of RNA biosynthetic process
transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
multicellular organism development
regulation of gene expression
Wnt signaling pathway
cell differentiation
osteoclast differentiation
regulation of cell proliferation
regulation of apoptotic process
camera-type eye development
negative regulation of apoptotic process
pigmentation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
cell fate commitment
regulation of osteoclast differentiation
bone remodeling
mast cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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