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Invitrogen
Caveolin 1 Monoclonal Antibody (SP43)
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FIGURE: 1 / 5
Caveolin 1 Antibody (MA5-16329) in ICC/IF
Immunofluorescence analysis of Caveolin 1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Caveolin 1 Monoclonal Antibody (SP43) (Product # MA5-16329) at 1:100 dilution in 0.1% BSA, incubated at 4 degre... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP43
Immunogen
Synthetic peptide corresponding to C-terminus of human caveolin-1 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.235 mg/mL
Amount
117.5 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung adenocarcinoma.
Target Information
Caveolin-1 or CAV1 is a scaffolding proteins within caveolar membranes. It interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-1 is also involved in the co-stimulatory signal essential for T-cell receptor (TCR) mediated T-cell activation. It can bind with DPP4 and induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations affecting the CAV1 gene can result in congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, or partial lipodystrophy/congenital cataracts, and neurodegeneration syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: caveolae; Caveolin-1; HGNC:1527; OTTHUMP00000196475
Gene Aliases: CAV; CAV1
Entrez Gene ID: (Human) 857
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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