Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 34
Cyclin D1 Antibody (MA5-16356) in ICC/IF
Immunofluorescence analysis of Cyclin D1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin D1 Rabbit Monoclonal Antibody (Product # MA1-39546) at 1:250 dilution in 0.1% BSA and incubated overnight ... View More
Product Details
MA5-16356
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Baboon,
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
proprietary
Class
Recombinant Monoclonal
Type
Antibody
Clone
SP4
Immunogen
Synthetic peptide from C-terminus of human cyclin D1.
Conjugate
Form
Liquid
Concentration
0.045 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma or mantle cell lymphoma.
Target Information
Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD-1; PRAD1 oncogene
Gene Aliases: AI327039; bcl-1; BCL1; CCND1; cD1; CycD1; Cyl-1; D11S287E; PRAD1; U21B31
UniProt ID: (Human) P24385, (Rat) P39948, (Mouse) P25322
Entrez Gene ID: (Human) 595, (Rat) 58919, (Mouse) 12443
transcription corepressor activity
protein kinase activity
protein binding
transcription factor binding
cyclin-dependent protein serine/threonine kinase regulator activity
enzyme binding
protein kinase binding
protein complex binding
histone deacetylase binding
proline-rich region binding
kinase activity
kinase activator
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
re-entry into mitotic cell cycle
positive regulation of protein phosphorylation
transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
lactation
response to iron ion
response to X-ray
response to organonitrogen compound
positive regulation of G2/M transition of mitotic cell cycle
negative regulation of Wnt signaling pathway
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
mitotic G1 DNA damage checkpoint
response to magnesium ion
response to estradiol
response to vitamin E
Leydig cell differentiation
mammary gland epithelial cell proliferation
positive regulation of mammary gland epithelial cell proliferation
response to drug
response to estrogen
response to leptin
fat cell differentiation
response to ethanol
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
cell division
response to corticosterone
response to calcium ion
canonical Wnt signaling pathway
mammary gland alveolus development
response to UV-A
negative regulation of cell cycle arrest
liver regeneration
liver development
positive regulation of cell proliferation
response to organic substance
response to organic cyclic compound
Wnt signaling pathway
organ regeneration
regulation of protein kinase activity
response to steroid hormone
response to glucocorticoid
regulation of cell cycle
cellular response to organic substance
regulation of G1/S transition of mitotic cell cycle
regulation of transcription, DNA-templated
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support