Invitrogen

CD8 Monoclonal Antibody (YCATE55.9), PE

Name: CD8 Monoclonal Antibody (YCATE55.9), PE
Brand: Invitrogen
SKU: MA5-16988

查看其他472个CD8抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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CD8 Antibody (MA5-16988) in Flow

Canine PBMCs were stained with monoclonal CD8 PE antibody (Product # MA5-16988) for 30 min on ice with indicated concentrations. After stanning, cells were washed by PBS with 5% heat inactivated calf serum + 5mM EDTA. Data were acquired immediately after resuspending cells in PBS with 1% formaldehyde. Total viable lymphocytes were gated for analysis. Data courtesy of Thermo Scientific KOL Program.

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产品信息

MA5-16988

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Neat

产品规格

种属反应

Dog

宿主/亚型

Rat / IgG1

分类

Monoclonal

类型

Antibody

克隆号

YCATE55.9

抗原

Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Lyophilized

浓度

Conc. Not Determined

纯化类型

Protein G

保存液

PBS with 1% BSA, 5% sucrose

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2538461

产品详细信息

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobbold et al. 1994).

靶标信息

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

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