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Invitrogen
IL5RA Monoclonal Antibody (26815), PE
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FIGURE: 1 / 2
IL5RA Antibody (MA5-23621) in Flow
Flow cytometry of IL5RA in HEK293 human embryonic kidney cell line transfected with (A) human IL‚5 Rα/CD125 or (B) irrelevant transfectants and eGFP. Samples were incubated in IL5RA monoclonal antibody (Product # MA5-23621). Quadrant markers were set based on control antibody staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-23621
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
26815
Immunogen
Sf 21-derived recombinant human IL-5R alpha/CD125 Asp21-Arg335
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with ≤ 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) IL-5 R beta, rhIL-9 R, rhIL-4 R, recombinant mouse IL-5 R alpha, or rhIL-13 R alpha 1.
Target Information
Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD125; CDw125; IL-5 receptor subunit alpha; Interleukin-5 receptor subunit alpha
Gene Aliases: IL5R; IL5RA
Entrez Gene ID: (Human) 3568
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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