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Invitrogen
SOX17 Monoclonal Antibody (OTI3B10)
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FIGURE: 1 / 10
SOX17 Antibody (MA5-24885) in ICC/IF
Immunofluorescence analysis of Transcription factor SOX-17 was performed using 70% confluent log phase iPSC and iPSC differentiated to definitive endoderm cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOX17 Monoclonal Antibody (OTI3B10) (Product # MA5-24885) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) ... View More
Product Details
MA5-24885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3B10
Immunogen
Human recombinant protein fragment corresponding to amino acids 177-414 of SOX17 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SOX17 (transcription factor SOX-17) belongs to the SRY-related HMB box family of transcription factors. It acts as a transcription regulator that binds target promoter DNA and bends the DNA. It binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. SOX17 modulates transcriptional regulation via WNT3A and inhibits Wnt signaling. It promotes degradation of activated CTNNB1. SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. Mutations in the gene can result in vesicoureteral reflux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: SRY (sex determining region Y)-box 17; SRY-box 17; SRY-related HMG-box transcription factor SOX17; Transcription factor SOX-17; unnamed protein product
Gene Aliases: PPH7; SOX17; VUR3
UniProt ID: (Human) Q9H6I2
Entrez Gene ID: (Human) 64321
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
angiogenesis
vasculogenesis
metanephros development
endoderm formation
endodermal cell fate specification
inner cell mass cellular morphogenesis
heart looping
cardiogenic plate morphogenesis
embryonic heart tube morphogenesis
outflow tract morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatogenesis
gastrulation
endoderm development
endodermal cell fate determination
heart development
tissue development
gene expression
positive regulation of gene expression
Wnt signaling pathway
rostrocaudal neural tube patterning
signal transduction involved in regulation of gene expression
negative regulation of Wnt signaling pathway
negative regulation of cell growth
protein destabilization
embryonic heart tube development
endodermal cell differentiation
cell migration involved in gastrulation
response to alkaloid
regulation of cell differentiation
positive regulation of protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of embryonic development
embryonic organ development
embryonic foregut morphogenesis
anatomical structure formation involved in morphogenesis
stem cell differentiation
stem cell fate specification
protein stabilization
endocardium formation
cardiac cell fate determination
heart formation
endocardial cell differentiation
common bile duct development
gall bladder development
endodermal digestive tract morphogenesis
regulation of stem cell proliferation
ureter development
negative regulation of canonical Wnt signaling pathway
positive regulation of endodermal cell differentiation
cellular response to leukemia inhibitory factor
regulation of stem cell division
regulation of cardiac cell fate specification
positive regulation of stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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