Invitrogen

CHAC1 Monoclonal Antibody (OTI1E2)

4个已发表图片

2 篇参考文献

查看其他11个CHAC1抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (10)
已发表图片 (4)

CHAC1 Antibody in Immunocytochemistry (ICC/IF)

CHAC1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

CHAC1 Antibody (MA5-26311) in ICC/IF

Immunofluorescent analysis of CHAC1 in COS7 cells. Cells were transfected with a plasmid overexpressing CHAC1 and probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunocytochemistry (ICC/IF)

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHAC1 Antibody in Western Blot (WB)

CHAC1 Antibody in Western Blot (WB)

CHAC1 Antibody in Western Blot (WB)

CHAC1 Antibody in Western Blot (WB)

CHAC1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CHAC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CHAC1 in COS7 cells. Cells were transfected with a plasmid overexpressing CHAC1 and probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CHAC1 monoclonal antibody (Product # MA5-26311).

CHAC1 Antibody in Western Blot (WB) — Western blot analysis of CHAC1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with CHAC1 (Product # MA5-26311) monoclonal antibody.

CHAC1 Antibody in Western Blot (WB) — Figure 3. NCTD increases the intracellular Fe 2+ content in SKOV3 and OVCAR-3 cells. SKOV3 and OVCAR-3 cells were pre-incubated with 1 uM Fer-1 for 1 h. The SKOV3 and OVCAR-3 cells were then treated with 10 and 20 uM NCTD for an additional 24-h time period. Pre-incubation with Fer-1 reduced the NCTD-induced upregulation of MDA contents in (A) SKOV3 and (B) OVCAR-3 cells. Fer-1 decreased the NCTD-induced elevation in the Fe 2+ contents in (C) SKOV3 and (D) OVCAR-3 cells. Reverse transcription-quantitative PCR analysis demonstrated that the PTGS2 and CHAC1 mRNA levels were decreased in the (E and G) SKOV3 and (F and H) OVCAR-3 cells, pre-incubated with Fer-1. Western blot analysis indicated that the expression of PTGS2 and CHAC1 was enhanced in (I) SKOV3 and (J) OVCAR-3 cells treated with NCTD, compared with that of the control. One-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *P<0.05, **P<0.01 and ***P<0.001 vs. control (DMSO); # P<0.05, ## P<0.01 and ### P<0.001 vs. NCTD. NCTD, norcantharidin; Fer-1, ferrostatin-1; MDA, malonaldehyde; Fe 2+ , ferrous ion; PTGS2, prostaglandin-endoperoxide synthase 2; CHAC1, glutathione-specific gamma-glutamylcyclotransferase 1.

CHAC1 Antibody in Western Blot (WB) — Figure 5. NCTD suppresses tumor growth in vivo . (A) Representative tumor images. Treatment with 100 and 200 mg/kg NCTD for 4 weeks significantly suppressed (B) tumor volume and (C) weight. (D) Treatment with 100 and 200 mg/kg NCTD significantly increased the GSH content in the tumors. The (E) MDA, (F) ROS and (G) Fe 2+ contents were significantly decreased following treatment with 100 and 200 mg/kg NCTD for 4 weeks. (H) The results of western blot analysis demonstrated that NCTD reduced the NRF2, HO-1, xCT and GPX4 protein levels, elevating the expression of PTGS2 and CHAC1 in SKOV3-derived xenografts. One-way ANOVA followed by Tukey's post hoc test was used for statistical anlaysis. *P<0.05, **P<0.01 and ***P<0.001 vs. control. Con, control; NCTD, norcantharidin; ROS, reactive oxygen species; MDA, malondialdehyde; Fe 2+ , ferrous ion; NRF2, nuclear factor erythroid 2-related 2; CHAC1, glutathione-specific gamma-glutamylcyclotransferase 1; HO-1, heme oxygenase 1; xCT, solute carrier family 7 member 11; GPX4, glutathione peroxidase 4.

CHAC1 Antibody in Western Blot (WB) — The effects of ATF4 on Sev-induced ferroptosis in glioma cells. U87 and U251 cells were transfected with ATF4 siRNA or negative control sequences, followed by treatment with 5.1% Sev for 2 h. Cell viability was detected using CCK-8 assay in U87 (A) and U251 cells (B) . Fe2+ concentrations were determined by colorimetric assay in U87 and U251 cells (C) . ROS assay combined with flow cytometry was used to observe the content of ROS generation in U87 and U251 cells (D) ; the ratio of ROS generation was calculated (E) . The expression of ferroptosis-associated protein CHAC1, GPX4, ferritin, and transferrin in U87 and U251 cells was detected using Western blotting; GAPDH was used as the internal control (F, G) . Data were expressed as mean +- standard deviation. *p < 0.05, **p < 0.01, ***P<0.0001, compared with control.

CHAC1 Antibody in Western Blot (WB) — Induction of ferroptosis reversed the effects of ATF4 suppression on Sev-induced ferroptosis in glioma cells. Ferroptosis inducer Erastin was used to incubate in Sev-treated and ATF4-suppressed U87 and U251 cells. Cell viability was detected using CCK-8 assay in U87 (A) and U251 cells (B) . Fe2+ concentrations were determined by colorimetric assay in U87 and U251 cells (C) . ROS assay combined with flow cytometry was used to observe the content of ROS generation in U87 and U251 cells (D) ; the ratio of ROS generation was calculated (E) . The expression of ATF4 and CHAC1 in U87 and U251 cells was detected using Western blotting; GAPDH was used as the internal control (F, G) . Data were expressed as mean +- standard deviation. *p < 0.05, **p < 0.01, compared with control.

Immunogen image

CHAC1 Monoclonal Antibody (OTI1E2)

Immunogen Image CHAC1 Monoclonal Antibody (OTI1E2)

产品信息

MA5-26311

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:4,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:150

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI1E2

抗原

Full length human recombinant protein of CHAC1 produced in HEK293T cell

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Blocks Notch protein; Botch; Cation transport regulator-like protein 1; Gamma-GCG 1; Glutathione-specific gamma-glutamylcyclotransferase 1

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基因别名: BOTCH; CHAC1

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Entrez Gene ID: (Human) 79094

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