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Invitrogen
Cullin 4B Monoclonal Antibody (OTI1C4)
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FIGURE: 1 / 6
Cullin 4B Antibody (MA5-26445) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded carcinoma of human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a CUL4B monoclonal antibody (Product # MA5-26445).
Product Details
MA5-26445
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1C4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of CUL4B produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL-4B; cul4b; Cullin-4B; ubiquitin E3 ligase; unnamed protein product
Gene Aliases: 2700050M05Rik; AA409770; CUL-4B; CUL4B; KIAA0695; mKIAA0695; MRXHF2; MRXS15; MRXSC; SFM2
UniProt ID: (Human) Q13620, (Mouse) A2A432
Entrez Gene ID: (Dog) 492102, (Human) 8450, (Mouse) 72584
G1/S transition of mitotic cell cycle
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
gene expression
proteasomal protein catabolic process
protein ubiquitination
cellular response to UV
ribosome biogenesis
positive regulation of protein catabolic process
astrocyte differentiation
UV-damage excision repair
positive regulation of G1/S transition of mitotic cell cycle
cell cycle
neuron projection development
histone H2A monoubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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