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Invitrogen
Dicer Monoclonal Antibody (OTI1G8)
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FIGURE: 1 / 2
Dicer Antibody (MA5-26507) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a DICER1 monoclonal antibody (Product # MA5-26507).
Product Details
MA5-26507
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1G8
Immunogen
Human recombinant protein fragment corresponding to amino acids 1666-1922 of DICER1 produced in E.coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antiviral isoform of Dicer; DCR; DCR-1; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi; unnamed protein product; variant of Dicer1
Gene Aliases: aviD; DCR1; DICER; DICER1; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; KIAA0928; MNG1; RMSE2
UniProt ID: (Human) Q9UPY3
Entrez Gene ID: (Human) 23405
nucleotide binding
nucleic acid binding
RNA binding
double-stranded RNA binding
helicase activity
nuclease activity
endonuclease activity
endoribonuclease activity
ribonuclease III activity
deoxyribonuclease I activity
protein binding
ATP binding
hydrolase activity
endoribonuclease activity, producing 5'-phosphomonoesters
protein domain specific binding
siRNA binding
metal ion binding
regulatory RNA binding
pre-miRNA binding
negative regulation of transcription from RNA polymerase II promoter
apoptotic DNA fragmentation
RNA processing
miRNA metabolic process
negative regulation of Schwann cell proliferation
negative regulation of gene expression
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of Schwann cell differentiation
tRNA catabolic process
posttranscriptional gene silencing
nerve development
production of siRNA involved in RNA interference
gene silencing by RNA
pre-miRNA processing
positive regulation of myelination
peripheral nervous system myelin formation
negative regulation of tumor necrosis factor production
production of miRNAs involved in gene silencing by miRNA
neuron projection morphogenesis
regulation of multicellular organismal process
small RNA loading onto RISC
global gene silencing by mRNA cleavage
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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