Invitrogen

PLAC8 Monoclonal Antibody (OTI2E10)

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1 篇参考文献

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问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (6)
已发表图片 (1)

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PLAC8 Antibody (MA5-27363) in IHC (P)

Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLAC8 monoclonal antibody (Product # MA5-27363) at a dilution of 1:500.

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLAC8 Antibody in Western Blot (WB)

PLAC8 Antibody in Western Blot (WB)

PLAC8 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLAC8 monoclonal antibody (Product # MA5-27363) at a dilution of 1:500.

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLAC8 monoclonal antibody (Product # MA5-27363) at a dilution of 1:500.

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLAC8 monoclonal antibody (Product # MA5-27363) at a dilution of 1:500.

PLAC8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human tonsil tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLAC8 monoclonal antibody (Product # MA5-27363) at a dilution of 1:500.

PLAC8 Antibody in Western Blot (WB) — Western blot analysis of PLAC8 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PLAC8 (Product # MA5-27363) monoclonal antibody.

PLAC8 Antibody in Western Blot (WB) — Western blot analysis of PLAC8 in human liver cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with PLAC8 (Product # MA5-27363) monoclonal antibody at a dilution of 1:500.

PLAC8 Antibody in Western Blot (WB) — Inhibition of PLAC8 expression enhanced CB-ECFC NOX4-mediated angiogenic signalling in hypoxia. CB-ECFCs were transfected with either PLAC8-targeting siRNA or non-targeting SCR control for 24 h prior to culture in hypoxia or normoxia for 48 h. ( A ) Confirmation of PLAC8 KD by Western blot with normalisation to beta-actin as reference control. ( B ) Tube area confirmed by Matrigel tubulogenesis assay. ( C - E ) Protein expression of NOX4, Nrf2 and eNOS by Western blot with normalisation to beta-actin as reference control. Production of ( F ) superoxide confirmed using DHE staining and ( G ) hydrogen peroxide using a commercial assay with Image-J quantification. For scatter plots, data were mean +- SEM; n = 9 combined from three CB-ECFC clones; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus relevant SCR control, unpaired Student's t -test or one-way ANOVA with Bonferroni post-hoc testing.

Immunogen image

PLAC8 Monoclonal Antibody (OTI2E10)

Immunogen Image PLAC8 Monoclonal Antibody (OTI2E10)

产品信息

MA5-27363

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:500

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产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2E10

抗原

Full length human recombinant protein of PLAC8 produced in E.coli

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

PLAC8 is a protein coding gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Placenta-specific gene 8 protein; Protein C15

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基因别名: BM-004; PLAC8

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Entrez Gene ID: (Human) 51316

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