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Invitrogen
14-3-3 beta/zeta Recombinant Rabbit Monoclonal Antibody (JJ084-3)
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14-3-3 beta/zeta Antibody (MA5-32448) in WB
Western blot was performed using Anti-14-3-3 beta/zeta Recombinant Rabbit Monoclonal Antibody (JJ084-3) (Product # MA5-32448) and a 28 kDa band corresponding to 14-3-3 protein beta/zeta was observed across the cell lines and tissues tested. Membrane enriched extracts (10 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), PC-3 (Lane 3), A549 (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Brain (Lane 7), Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved pr... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-32448
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JJ084-3
抗原
Recombinant protein within Human 14-3-3 aa 1-122.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3d; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Prepronerve growth factor RNH-1; Protein 1054; Protein kinase C inhibitor protein 1; SEZ-2; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; YWHAE
基因别名: 1110013I11Rik; 1300003C17Rik; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; Msfs1; YWHAB; YWHAZ
UniProt ID: (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P63101, (Rat) P63102
Entrez Gene ID: (Mouse) 54401, (Rat) 56011, (Mouse) 22631, (Rat) 25578
transcription corepressor activity
protein binding
protein C-terminus binding
enzyme binding
protein domain specific binding
protein complex binding
histone deacetylase binding
phosphoserine binding
phosphoprotein binding
transcription factor binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
poly(A) RNA binding
scaffold/adaptor protein
protein targeting
negative regulation of protein dephosphorylation
positive regulation of catalytic activity
negative regulation of transcription, DNA-templated
cytoplasmic sequestering of protein
protein heterooligomerization
histamine secretion by mast cell
protein targeting to mitochondrion
regulation of cell death
response to drug
establishment of Golgi localization
Golgi reassembly
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