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Invitrogen
Actin Recombinant Rabbit Monoclonal Antibody (JJ09-29)
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图: 1 / 10
Actin Antibody (MA5-32479) in ICC/IF
Immunofluorescence analysis of ACTA1 was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Actin Recombinant Rabbit Monoclonal Antibody (Product # MA5-32479) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 c... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-32479
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Zebrafish
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JJ09-29
抗原
Synthetic peptide within Human Actin aa 45-80.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.0685 mg/mL
规格
106.85 µg
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ACT2; actin 2; actin 7; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; AtACT7; Atcb; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; FIZ2; FRIZZY AND KINKED SHOOTS 2; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; pan actin; RP5-1068B5.2; sma; smooth muscle alpha-actin; Smooth muscle gamma-actin
基因别名: 0610041G09Rik; a-SMA; AA959943; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; beta-actin; E430023M04Rik; E51; GIG46; SMalphaA; SMGA; wu:fb63d03
nucleotide binding
protein binding
ATP binding
protein kinase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
nitric-oxide synthase binding
ATPase activity
myosin binding
structural constituent of cytoskeleton
ubiquitin protein ligase binding
identical protein binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
skeletal muscle thin filament assembly
skeletal muscle fiber development
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
muscle contraction
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
retina homeostasis
axonogenesis
substantia nigra development
ATP-dependent chromatin remodeling
retina development in camera-type eye
platelet aggregation
cellular response to electrical stimulus
metabolic process
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
negative regulation of apoptotic process
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
actin-mediated cell contraction
response to drug
response to ethanol
sarcomere organization
response to calcium ion
biological_process
blood circulation
positive regulation of gene expression
mesenchyme migration
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