Invitrogen

BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3)

Name: BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3)
Brand: Invitrogen
SKU: MA5-32486
Availability: InStock

高级验证

1 篇参考文献

查看其他43个BMI-1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (10)
高级验证 (1)

BMI-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

BMI-1 Antibody (MA5-32486) in ICC/IF

Immunocytochemical analysis of BMI-1 in SW480 cells using a BMI-1 Monoclonal antibody (Product # MA5-32486) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

BMI-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

BMI-1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Bmi1; polycomb group ring finger 4 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3) (Product # MA5-32486). {KD}

Immunogen image

Immunogen Image BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3)

产品信息

MA5-32486

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JJ093-3

抗原

Synthetic peptide within Human Bmi1 aa 282-326.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2809763

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B lymphoma Mo-MLV insertion region 1; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

查看更多收起

基因别名: BMI1; PCGF4; RNF51

查看更多收起

Entrez Gene ID: (Rat) 307151

查看更多收起

功能

chromatin binding ubiquitin-protein transferase activity zinc ion binding sequence-specific DNA binding RING-like zinc finger domain binding ubiquitin-protein transferase activator activity

参与通路