Invitrogen

Ub-K63 Recombinant Rabbit Monoclonal Antibody (JM09-67)

Name: Ub-K63 Recombinant Rabbit Monoclonal Antibody (JM09-67)
Brand: Invitrogen
SKU: MA5-32573
Availability: InStock

查看其他4个Ub-K63抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (7)

Ub-K63 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

Ub-K63 Antibody (MA5-32573) in ICC/IF

Immunocytochemical analysis of Ubiquitin in 293T cells using a Ubiquitin Monoclonal antibody (Product # MA5-32573) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Ub-K63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Ub-K63 Recombinant Rabbit Monoclonal Antibody (JM09-67)

产品信息

MA5-32573

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM09-67

抗原

Synthetic peptide within Human K63-linkage Specific Ubiquitin aa 36-85.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2809850

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 60S ribosomal protein L40; CEP52; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin C; Ubiquitin carboxyl extension protein 80; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-60S ribosomal protein L40; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein

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基因别名: 0610006J14Rik; 2700054O04Rik; AI194771; AL033289; D8Ertd21e; Gm1863; RPS27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2