Invitrogen

Gata-4 Recombinant Rabbit Monoclonal Antibody (JA11-41)

查看其他27个GATA4抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)

Gata-4 Antibody in Immunocytochemistry (ICC/IF)

Gata-4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

Gata-4 Antibody (MA5-32678) in ICC/IF

Immunocytochemical analysis of Gata-4 in PC-3M cells using a Gata-4 Monoclonal antibody (Product # MA5-32678) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Gata-4 Antibody in Immunocytochemistry (ICC/IF)

Gata-4 Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Gata-4 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Gata-4 in PC-3M cells using a Gata-4 Monoclonal antibody (Product # MA5-32678) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Gata-4 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of Gata-4 in Hela cells using a Gata-4 Monoclonal Antibody (Product # MA5-32678) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunogen image

Gata-4 Recombinant Rabbit Monoclonal Antibody (JA11-41)

Immunogen Image Gata-4 Recombinant Rabbit Monoclonal Antibody (JA11-41)

产品信息

MA5-32678

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫细胞化学 (ICC/IF)

1:250

-

流式细胞分析 (Flow)

1:50-1:100

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JA11-41

抗原

Synthetic peptide within Human GATA 4 aa 5-54.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DNA-binding protein GATA-GT2; GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4

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基因别名: Gata-4; GATA4

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UniProt ID: (Mouse) Q08369, (Rat) P46152

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Entrez Gene ID: (Mouse) 14463, (Rat) 54254

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding NFAT protein binding co-SMAD binding DNA-binding transcription factor

in utero embryonic development gastrulation with mouth forming second endoderm formation heart looping heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis atrioventricular valve formation mitral valve formation tricuspid valve formation endocardial cushion development cardiac right ventricle morphogenesis ventricular cardiac muscle tissue development cardiac septum development ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cell-cell signaling spermatogenesis heart development male gonad development response to mechanical stimulus regulation of gene expression negative regulation of autophagy positive regulation of vascular endothelial growth factor production cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway positive regulation of BMP signaling pathway response to retinoic acid embryonic heart tube development embryonic heart tube anterior/posterior pattern specification tube morphogenesis skeletal muscle cell differentiation atrioventricular canal development response to drug response to estrogen positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract morphogenesis embryonic morphogenesis embryonic foregut morphogenesis cardiac muscle tissue development cardiac muscle cell differentiation Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway transdifferentiation SMAD protein signal transduction atrial septum morphogenesis lung morphogenesis lung lobe formation diaphragm morphogenesis intestinal epithelial cell differentiation vasculogenesis involved in coronary vascular morphogenesis cell growth involved in cardiac muscle cell development cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development regulation of cardiac muscle cell contraction response to vitamin A positive regulation of cardioblast differentiation cellular response to glucose stimulus