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Invitrogen
DNMT1 Recombinant Rabbit Monoclonal Antibody (1K5K8)
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FIGURE: 1 / 6
DNMT1 Antibody (MA5-35340) in WB
Western blot analysis of DNMT1 in extracts of various cell lines. Samples were incubated with DNMT1 Monoclonal antibody (Product # MA5-35340) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35340
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1K5K8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_0013701)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MPARTAPARV PTLAVPAISL PDDVRRRLKD LERDSLTEKE CVKEKLNLLH EFLQTEIKNQ LCDLETKLRK EELSEEGYLA KVKSLLNKDL SLENGAHAYN
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase (cytosine-5) 1; DNA methyltransferase HsaI; DNA methyltransferase I; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; DNA MTase RnoIP; Dnmt1; FLJ16293; m.RnoIP; MCMT; MGC104992
Gene Aliases: AIM; CXXC9; DNMT; DNMT1; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD2; MTase; Uim
UniProt ID: (Mouse) P13864
Entrez Gene ID: (Rat) 84350, (Mouse) 13433
DNA binding
chromatin binding
double-stranded DNA binding
RNA binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
transcription factor binding
methyltransferase activity
zinc ion binding
methyl-CpG binding
S-adenosylmethionine-dependent methyltransferase activity
DNA-methyltransferase activity
protein domain specific binding
estrogen receptor binding
histone deacetylase binding
unmethylated CpG binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
catalytic activity
transferase activity
metal ion binding
DNA metabolism protein
DNA methyltransferase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
transcription, DNA-templated
regulation of transcription, DNA-templated
brain development
aging
response to heat
response to toxic substance
response to organic substance
response to ionizing radiation
maintenance of DNA methylation
response to lead ion
DNA methylation on cytosine within a CG sequence
regulation of gene expression
positive regulation of gene expression
response to activity
gene silencing
chromatin modification
neuron differentiation
response to caffeine
response to nutrient levels
response to estradiol
response to lipopolysaccharide
DNA methylation on cytosine
response to vitamin A
response to testosterone
cellular response to platelet-derived growth factor stimulus
response to antidepressant
regulation of cell proliferation
response to drug
DNA hypermethylation
response to ethanol
negative regulation of transcription, DNA-templated
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
S-adenosylmethionine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
cellular response to amino acid stimulus
cellular response to lead ion
cellular response to transforming growth factor beta stimulus
C-5 methylation of cytosine
cellular response to nerve growth factor stimulus
metabolic process
methylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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