Search
Invitrogen
Calpain 2 Recombinant Rabbit Monoclonal Antibody (9Y7I1)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
Calpain 2 Antibody (MA5-35436) in WB
Western blot analysis of Calpain 2 in extracts of various cell lines. Samples were incubated with Calpain 2 Monoclonal antibody (Product # MA5-35436) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35436
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9Y7I1
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Calpain 2 (P17655)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.5 mg/mL
Amount
150 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LWTKIQKYQK IYREIDVDRS GTMNSYEMRK ALEEAGFKMP CQLHQVIVAR FADDQLIIDF DNFVRCLVRL ETLFKIFKQL DPENTGTIEL DLISWLCFSV L
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 80 kDa M-calpain subunit; Ca2-activated neutral protease subunit; calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; calpain M-type; calpain, large polypeptide L2; calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; millimolar-calpain; unnamed protein product
Gene Aliases: CALP80; CANP2; CANPL2; CANPml; Capa-2; Capa2; m-calpain; m-calpin; mCANP
UniProt ID: (Human) P17655, (Rat) Q07009, (Mouse) O08529
Entrez Gene ID: (Human) 824, (Rat) 29154, (Mouse) 12334
response to hypoxia
proteolysis
female pregnancy
response to mechanical stimulus
positive regulation of cardiac muscle cell apoptotic process
protein autoprocessing
protein catabolic process
regulation of interleukin-6 production
cellular response to interferon-beta
response to hydrogen peroxide
behavioral response to pain
synaptic vesicle endocytosis
regulation of cytoskeleton organization
negative regulation of focal adhesion assembly
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
vascular endothelial cell response to laminar fluid shear stress
vascular endothelial cell response to oscillatory fluid shear stress
protein catabolic process at postsynapse
positive regulation of myoblast fusion
positive regulation of phosphatidylcholine biosynthetic process
blastocyst development
myoblast fusion
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support